Abstract: The effects of Penshibao, one of plant nutritional regulator on the seed germination and seedlings growth of peanut under different temperatures were studied. Peanut seeds soaked with different concentrations of Penshibao (P.S.B) increased germination rate, the length of radical as well as its diameter under different temperatures. The germination ratio was in the highest when concentration was 1 000 mg/L; the increasements of radical length and its diameter were the most significant when concentration was 300 mg/L. P.S.B also stunted seedlings growth, increased branching numbers, the mass of the aerial part, and the content of leaf chlorophyll, protein and dissolvable sugar.It also promoted the leaf nitrate reductase activity and the root activity of seedling. For aerial part ,the better concentration was 300-500 mg/L .Besides, it also increased the elongation of taproot,its diameter, amounts of nodules and seedling fresh mass. The best concentration for the elongation of taproot was 100-300 mg/L.