Abstract: The genomic DNA polymorphism of five mutants, selected from rice variety Texianzhan13 seeds which had been flown on a recoverable satellite, was analysized by using 299 rice SSR(simple sequence repeats) primer pairs selected throughout the 12 chromosome of rice genome. The results indicated that the polymorphism among mutants and origional variety was various. The polymorphic frequency, based on the ratio of number of primers amplifying polymorphic band pattern to the number of amplifying any bands, was 0.35% to 2.47% in different mutants.