非洲山毛豆叶片中鱼藤酮的提取方法

Extracting Methods of Rotenone in Leaves of Tephrosia vogelii Hook f.

摘要: 用索氏、冷浸和振荡抽提3种方法和6种不同的溶剂对非洲山毛地片进行了提取研究，并用HPLC测定了提取物和植物叶片干粉中鱼藤酮的含量。结果表明，用氯仿振荡抽提紫花品种叶片得到的粗提物中鱼藤酮的含量最高，w为9．70％；丙酮索氏抽提紫花品种叶片所求得的植物干粉中鱼藤酮的含量最高，w为0．72%。中等极性溶剂加氯仿、苯、乙酸乙酯、丙酮等对鱼藤酮的提取率较高，建议实验室内提取溶剂用氯仿或丙酮，工厂化生产时用苯较好。

Abstract: Three kinds of extracting methods and six kinds of solvents were applied to extract the leaves of Tephrosia vogelii Hook f.. the content of rotenone in the extracts and dry powder of leaves were detected by HPLC. It showed that the content of rotenone in the crude extracts extracted by chloroform with vibrating extraction from purple variety was the highest to 9.70% , and the respond content of rotenone in dry powder of leaves of T. vogelii extracted by acetone with Sohxlet from purple variety was the highest to 0.72%. Moderate polarity solvents such as chloroform, benzene, acetone and ethyl acetate were proper to extract rotenone, while chloroform or acetone used in laboratory and benzene used in factory were appropriate.