Abstract: Eleven samples of MDCK cell lines collected from various university laboratories, epidemic prevention stations and quarantine bureaus within China since 1997. Utilizing 4 stretches of nucleotide sequences reported for the VP 2 gene of canine parvovirus as primers, the DNA of the samples were screened with a nested PCR procedure. The PCR amplified product was subjected to electrophoretic analysis in 0.01 g/mL agarose, cloned in a PGEM T vector and the nucleotide sequence determined. The findings revealed the presence of a vertically transmitted canine parvovirus. Partial sequencing of the virus genome showed 91% nucleotide homology with the canine parvovirus, strain CPV N. This is the first case report of canine parvovirus in MDCK cell lines in our country.