从烟草悬浮细胞制备辅酶Q10的初步研究

基金项目: 广东省自然科学基金!960452

Studies on the Preparation of Coenzyme Q_(10) from Tobacco (Nicotiana tobacum L.) Cells in Suspension Culture

摘要: 采用细胞悬浮的方法，从４个烟草品种（系）中筛选出辅酶Ｑ１０含量较高的黄花大金元，以其悬浮培养细胞为材料，比较了几种植物生长调节剂对细胞生长和辅酶Ｑ１０含量的影响。结果表明，０．１￣０．２ｍｇ／Ｌ的ＢＡ和ＫＴ对烟草细胞有显著的促进作用，但使辅酶Ｑ１０含量略有降低；０．５￣８．０ｍｇ／Ｌ的２，４－Ｄ可显著提高辅酶Ｑ１０含量，但当其浓度高时对细胞生长不利；ＩＡＡ和ＮＡＡ对烟草细胞生长和辅酶Ｑ１０含量均无
- 烟草 /
- 细胞悬浮培养 /
- 辅酶Q10
Abstract: A tobacco cell stain of HHDJY was selected by suspension cultare from 4 tobacco strains cultivated in South China on the level of CoQ10. By using this tobacco cell strain, the effectS of several plant growth regulation on the cell growth and content of CoQ10 were studied. The growth of the cells was greatly enhanced by 0.1 ~ 0. 2 mg/L BA and KT. 2,4-D could increase the content of CoQ10 but becampe unfavorable to cell growth when the concentration was high. IAA and NAA had no effect on cell growth and content of CoQ10. Morcover, CoQ10 crystal was obtained from suspension cells with acetone abstraction,petrolembenzine extraction and silica chromatography. The recovery of CoQ10 was 59. 2%, and the purity reached 97. 7 %.
- tobacco cells /
- suspension culture /
- coenzyme Q_(10)