苦槛蓝抽提物对菜青虫生物活性的影响
Effects on the Bioactivity of the Extracts from Myoporum bontioides Against Imported Cabbage Worm (Pieris rapae)
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摘要: 报道了苦槛蓝油抽提物对菜粉蝶幼虫拒食、毒杀和影响生长发育作用的生物活性。试验表明ρ＝１０ｇ／Ｌ的苦槛蓝叶、茎浸提物对菜粉蝶３龄幼虫的非选择性拒食率均在９０％以上，对４龄和５龄幼虫的拒依率分别为８０％￣８６％和４６％￣６５％．Ｐｏｔｔｅｒ喷雾处理７２ｈ后，ρ＝１０ｇ／Ｌ叶甲醇浸提物对３龄幼虫的校正死亡率可达８６．１，而ρ＝１０ｇ＝／Ｌ叶石油醚浸提物对４龄幼虫的校正死亡率则为８８．５％。用苦槛蓝叶氯仿Abstract: The significant antifeeding action and poisoning of the extracts from Myoporum bontioides against Pieris rapae L. were reported in this paper. The antifeeding rate with 1% methanolic extract andpetroleum ether extract from leaves and stems were above 90% against 3rd instar lareae, and 80% ~86% and 46% ~ 65% against 4th and 5th instar larvae of the insect pest in no-choice tests, respectively. After treatment with methanolic extract and petroleum ether extract in 72h, 86. 1% and 88. 5% ofmertality were obtained, respectively. The LC50 of the extract partitioned with chloroform and methanolfrom methanol extract against 4th instar larvae were 0. 63% and 1. 39%, respectively. In four days aftertreatment, with 1% chloroform extract partitioned from methanol extract, the average weight of 5th instarlarvac was 38. 05% of that in control, and the emergence rate of adult was 4.00% and the rate of growthinhibition was 88. 89%.
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Keywords:
- Myoporum bontioides /
- extract /
- Pieris rapae /
- bioactivity
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