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    宋延华 刘福安. 含有IBV S1基因的昆虫杆状病毒表达载体的构建[J]. 华南农业大学学报, 1998, (4): 1-4.
    引用本文: 宋延华 刘福安. 含有IBV S1基因的昆虫杆状病毒表达载体的构建[J]. 华南农业大学学报, 1998, (4): 1-4.
    shu
    THE CONSTRUCTION OF BACULOVIRUS TRANSFER VECTOR CONTAINING THE S1 GENE OF AVIAN INFECTIOUS BRONCHITIS VIRUS[J]. Journal of South China Agricultural University, 1998, (4): 1-4.
    Citation: THE CONSTRUCTION OF BACULOVIRUS TRANSFER VECTOR CONTAINING THE S1 GENE OF AVIAN INFECTIOUS BRONCHITIS VIRUS[J]. Journal of South China Agricultural University, 1998, (4): 1-4.
    shu

    含有IBV S1基因的昆虫杆状病毒表达载体的构建

    THE CONSTRUCTION OF BACULOVIRUS TRANSFER VECTOR CONTAINING THE S1 GENE OF AVIAN INFECTIOUS BRONCHITIS VIRUS

      摘要
    • 摘要: 以含有鸡传染性支气管炎病毒Ｈｏｌｔｅ株Ｓ１基因的重组质粒ｐＵＣ１８－Ｓ１．Ｈｏｌｔｅ和ｐＵＣ１８－Ｓ‘１．Ｈｏｌｔｅ为材料，分别构建了可表达ＩＢＶ Ｓ１基因的昆虫杆状病毒转移载体质粒ｐＳＸＩＶＶＩ＾＋Ｘ３－Ｓ１．Ｈｏｌｔｅ（含ＩＢＶ先导序列），ｐＳＸＩＶＶＩ＾＋Ｘ３／４－Ｓ１．Ｈｏｌｔｅ（ＩＢＶ先导序列为融合短肽），ｐＡｃＧＨＬ－Ｃ－Ｓ１，Ｈｏｌｔｅ。

       

      Abstract: The recombinant transfer plasmids pSXIVVI +X3 S1.Holte and pSXIVVI +X3/4 S1.Holte and pAcGHLT C S1.Holte were constructed by insertion of the cDNA of the S1 gene of avian infectious bronchitis virus (IBV) into transfer vector plasmids pSXIVVI +X3 and pSXIVVI +X3/4 and pAcGHLT C.

       

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