含有IBV S1基因的昆虫杆状病毒表达载体的构建

THE CONSTRUCTION OF BACULOVIRUS TRANSFER VECTOR CONTAINING THE S1 GENE OF AVIAN INFECTIOUS BRONCHITIS VIRUS

摘要: 以含有鸡传染性支气管炎病毒Ｈｏｌｔｅ株Ｓ１基因的重组质粒ｐＵＣ１８－Ｓ１．Ｈｏｌｔｅ和ｐＵＣ１８－Ｓ‘１．Ｈｏｌｔｅ为材料，分别构建了可表达ＩＢＶＳ１基因的昆虫杆状病毒转移载体质粒ｐＳＸＩＶＶＩ＾＋Ｘ３－Ｓ１．Ｈｏｌｔｅ（含ＩＢＶ先导序列），ｐＳＸＩＶＶＩ＾＋Ｘ３／４－Ｓ１．Ｈｏｌｔｅ（ＩＢＶ先导序列为融合短肽），ｐＡｃＧＨＬ－Ｃ－Ｓ１，Ｈｏｌｔｅ。

Abstract: The recombinant transfer plasmids pSXIVVI +X3 S1.Holte and pSXIVVI +X3/4 S1.Holte and pAcGHLT C S1.Holte were constructed by insertion of the cDNA of the S1 gene of avian infectious bronchitis virus (IBV) into transfer vector plasmids pSXIVVI +X3 and pSXIVVI +X3/4 and pAcGHLT C.