Abstract: Effects of mixture planting symbiosis on yield of 13 mixture planting symbioic colonies(named:mixture line rice) which were mixed by each two of 7 two-line hybrid rices were studied.The results showed the yield of most mixture-line rices lied between two reference combination's yield, but some significantly increased comparing with the reference combination's. It indicated that mixture planting symbiosis in the same crop existed supplementary effects or inhibited effects. As for its law, it was necessary to be further studied, in order to providing a theoretical basis for utilization in production.