植物青枯菌的血清分型研究

    THE SEROTYPES OF BACTERIAL WILT PATHOGEN (Pseudomonas solanacearum) IN PLANTS

    • 摘要: 以代表不同生化型的4个青枯假单胞菌的膜蛋白为免疫原制备了4个相应的抗血清,对我国植物青枯菌的血清分型进行了研究。琼脂双扩散试验结果表明:秋自我国6个省的19种寄主植物的212个青枯菌株可分为8个血清型,其中血清型Ⅰ为优势类群,包括了主要茄科植物等13种寄主的147个菌株,占全部供试菌株69%。

       

      Abstract: A study was conducted to determine the serological relationships between strains of Pseudomonas solanacerum in China. Four corresponding antisera were prepared to a kind of membrane protein extracted from cells of four strains representing different biotypes of P. solanacerum respectively. By using Ouchterlong double diffusion tests, 212 isolates from ninteen different hosts in six provinces and cities could be grouped into eight serotypes. It was demonstrated that 147 isolates of them belonged to serotype I which was the predominant one (69%) including thirteen various hosts. SDS PAGE profiles of mebrane proteins extracted from strains representing eight serotypes of P. solanacerum showed that there were some differences between serotypes.

       

    /

    返回文章
    返回