植物叶片超氧物歧化酶活性及其从象草叶片的提取

SUPEROXIDE DISMUTASE ACTIVITIY IN PLANT LEAVES AND ITS ISOLATION FROM Pennisetum purpureum SCHUMACH

摘要: 测定了华南农业大学校园内１８种常见植物叶片的超氧物歧化酶活性，发现鸡蛋花，白兰花，鸡蛋果，羊蹄甲和象草叶片的酶活性较高，以每ｇ鲜重计大于４００单位。经过热处理，硫酸铵沉淀和ＤＥＡＥ－纤维素柱层析等步骤，部分提纯了象草叶片超氧化歧化酶，并研究了部分性质。

Abstract: Superoxide dismutase (SOD) activity of leaves of 18 plants growing in South China Agricultural University were determined and reported in this paper. The leaves of 5 plants, Plumeria rubra cv. acutifolia, Magnolia denudata, Passiflora edulis,Bauhinia variegata and Pennisetum purpureum , had higher activity,above 400 units/g fresh weight.SOD was partially purified from Pennisetum purpureum leaves by heat treatment,(NH 4) 2SO 4 precipitation,and DEAE- cellulose chromatography,and its properties studied.