Abstract: The purification of inclusion body virus of insect and the preparation for the virus sample for electron microscope were tentatively studied in this paper. The pictures of inclusion body and its virion under electron microscope were shown clearly,and displayed themorphological character of virion and made it possible to identify insect virus with electron microscope. The special structure such as cellular and ditch-like concave propolyhedra, ultrastructure such as polyhedron massive proteinic Crystal,Polyhedron membrane,and herringbone Pattern of Protein subunit of baculiform virion and envelope of virion were also observed clearly.