广东兰花病毒病的鉴定和检测研究

STUDIES ON THE IDENTIFICATION AND DETECTION OF ORCHIDS VIRUSES IN GUANGDONG PROVINCE

摘要: 用建立的ＤＡＣ－ＥＬＩＳＡ法，检测了来自广东省７市１８个场场或兰圃的２０种兰共１５９个感病兰花样本，建兰花叶病毒（ＣｙＭＶ）和齿兰环斑病毒（ＯＲＳＶ）以及ＣｙＭＶ和ＯＲＳＶ复合感染分别在４０，２６和６个样本中检测到，分别占所检测样品的２５．２％，１６．４％和３．８％，占所检测的２０个兰种的５０％（１０／２０），３５％（７／１０）和１５％（３／２０），感染ＣｙＭＶ的兰种有墨兰，文心兰，大花惠兰（组培

Abstract: diseased orchid plant samples representing 20 species from 7 cities ofGuangdong provinee were tested for cymbidium mosaic virus(CyMV),odontoglossumringspot virus(ORSV),cucumber mosaic virus (CMV)and papaya ringspot virus(PRV)with direct antigen coating ELISA(DAC-ELISA).CyMV was detected in 40 samplesand ORSV was detected in 26 samples,which represented 25;2% and 1 6.4% of total159 samples surveyed, respectively.Mixed infections with both viruses occurred in 6samples, representing 3.8 % of the total samples surveyed.CyMV infections were de-detected in Cymbidium sinense,C.sp.,C.faberi,C.pendulum,C.ensifolium,Vanda sp.Oncidium,Dendrobium sp. ,Cattleya; ORSV infections were detected in Cymbidiumsinense,C. sp.Vanda sp. , Doris sp.,Eria sp,Phalaenopsis sp,double infections withboth viruses occurred in C.sp.,Oncidium sp,Vanda sp.