Abstract: Sinocalamus latiflora (Munro) McClure (Bamboo) has considerable economic importance. Its shoots can be eaten as food and its mature culms used as industrial material. Callus formation from explants of bamboo sprout sheath was induced on Murashige & Skoog basal medium supplemented with the 2, 4-D 4-8 mg/1 and kinetin 3 mg/l and agar 0.7%-0. 8%. Calli could grow continuously when they were subcuitured on the same medium or medium supplemented with other nutrients and hormones.