苜蓿抗性愈伤组织抗旱机理的初步研究
PRELIMINARY STUDIES ON THE MECHANISM FOR DROUGHT RESISTANCE OF ALFALFA RESISTANT CALLUS
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摘要: 以稳定抗生理干旱的愈伤组织为材料,研究了抗旱愈伤组织的抗旱机理.结果表明:抗旱愈伤组织的干物质含量明显高于对照.水势为-10.76bar,而对照愈伤组织的水热为-4.77bar.脯氨酸的含量为对照的4.4倍,K~-和蔗糖的含量比对照稍低.脱落酸的含量是对照的3.4倍,但远远低于对照愈伤组织在含20％聚乙二醇的培养基上逆境四天的脱落酸含量.电泳结果表明:抗性意伤组织中产生了新的蛋白质或多肽(分子量:17000,18000道尔顿),抗性愈伤组织细胞的膜特性得到了改善.提出了抗旱机理假设.Abstract: Studies were carried on the mechanism for drought resistance, using stable callus resistant to physiological drought as material. The results showed: The content of dry matter was obviously higher than that of CK. Its water potential was - 10. 76bar, while the water potential of CK was -4. 77bar. Proline content was 4. 4 times as high as that of CK,and the content of k as well as sucrose was slightly lower than that of CK The content of Abscisic Acid was 3. 4 times as high as that of CK.but obviously lower than that of CK stressed on medium containing 20% PEG for four days. New proteins or polypeptides (M. W 17000. 18000 dorton) were produced in the calllus. The property of cell membrane was improved. An assumption of the mechanism for drought resistance was put forward.
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