昆明小鼠单细胞胚胎体外培养的研究

基金项目: 国家自然科学基金

STUDY ON CULTURING KUMING MOUSE 1-CELL EMBRYOS IN VITRO

Zhang Shouquan Sun Tuo(Dept of animal science) (Dept. of animal scienceBeijing Agricultural Univeratity)

摘要: 本试验研究了葡萄糖、EDTA和小鼠血清对昆明小鼠单细胞胚体外发育的影响.结果发现:不含葡萄糖HECM-1组桑椹率为40.05％,对照组为8.14％;含EDTA的CZB组桑椹率为61.18％,对照组为47.44％;小鼠血清对昆明小鼠单细胞胚体外发育无促进作用。可见,葡萄糖抑制昆明小鼠单细胞胚胎体外发育;在不含葡萄糖的条件下,EDTA支持小鼠早期胚胎体外发育;无论有无葡萄糖小鼠血清对小鼠早期胚体外发育无促进作用。
- 小鼠 /
- 胚胎 /
- 体外培养 /
- 细胞
Abstract: This study was dealt with the effects of glucose and mouse serum and EDTA on Kuming mouse 1-cell embryo development in vitro. The results show that the precentage of morula embryos cultured in glucose free HECM-1 to 40. 05%, and the control only 8.14%, cultured in CZB containing 0.11mM EDTA is 61. 18%, the control 47. 44%. In conclusion, the presence of glucose in culture medium is detrimental to Kuming mouse 1-cell embryo development. EDTA supports mouse embryo developement in vitro in glucose -free medium also. Glucose lacked or not, mouse serum doesn't affected in vitro mouse embrvo development.
- Kuming mouse /
- 1-cell embryo /
- Glucose /
- EDTA /
- Mouse serum /
- Culture /
- In vitro