Abstract: This study was dealt with the effects of glucose and mouse serum and EDTA on Kuming mouse 1-cell embryo development in vitro. The results show that the precentage of morula embryos cultured in glucose free HECM-1 to 40. 05%, and the control only 8.14%, cultured in CZB containing 0.11mM EDTA is 61. 18%, the control 47. 44%. In conclusion, the presence of glucose in culture medium is detrimental to Kuming mouse 1-cell embryo development. EDTA supports mouse embryo developement in vitro in glucose -free medium also. Glucose lacked or not, mouse serum doesn't affected in vitro mouse embrvo development.