Abstract: Some blood protein (enzyme) polymorphisms in domestic fowls were determined by means of gel electrophoresis and some of them such as alkline phosphatase (Akp-1 and Akp-2). amylase (Amy-1 and Amy-2, esterase (Es-1), hemoglobin (Hb-1) and transferrin (Tf) were thought to be as biochemical genetic markers in the study. The origins and diversities of and relationships between 3 renowned native chicken breeds in Guangdong province, China,line diversities with in Yuehuang chicken and relationships between families of Line 102 of Yushuang chicken were studied with the above biochemical genetic markers. Amylase (Amy- 1 ) polymorphism and its relationships to the vitalities in fowls were also studied with special interests. The results showed that applying biochemical genetic markers to forming poultry lines as assistances,either applying several biochemical genetic markers was possible together or applying a certain biochemical genetic marker (e. g. Amy-1).