紫背金盘提取物对四种鳞翅目害虫作用活性的初步研究

    PRILIMINARY STUDIES ON THE BIOACTIVITY OF THE EXTRACTS FROM AJUGA NIPPONENSIS MAKINO AGAINST FOUR SPECIES OF LEPIDOPTEROUS INSECT PESTS

    • 摘要: 紫背金盘(A(?)(?)(Makine))提取物对菜青虫(P(?)(?)(L.))小菜蛾(P(?)xylostella(L.))斜纹夜蛾(Prode(?)ia Iit(?)(Fabricius))和粘虫(M(?) aeparola (Walker))的幼虫均有显著的抑制生长发育和拒食作用,甲醇提取物及其氯仿提取物对五龄菜青虫的拒食中浓度(AFC_(50))分别为0.31%和0.12%;抑制生长发育有效中量(ED_(50))分别为1.36mg/g和0.34mg/g.氯仿萃取物对3—4龄小菜蛾的AFC_(50)为0.23%,EC_(50)=为0.29%(饲喂法)和0.22%(点滴法)。杀虫活性成分是易溶于氯仿的弱极性物质,分离提纯工作仍在进行中。

       

      Abstract: A bioassay of the crude extracts,as well as its fractions of different organic solvens partitions of A.nipponensis against four species of insect pests were carried out in the laboratory.The results showed that the methanol extract and its chloroform fraction presented a significant activity of growth and ecdysis inhibitory as well as antifecdant action against the larvae of Pieris rapae L.Plutella xylostella L,Prodexia likra and Mythimna separata Walder.As to the 5th-instar larvae of P.rapae,their antifeeding median concentrations (AFC50)of the methanal extract and its chloroform fraction were 0.31 %and 0.12%,respectively by the methed of the leaf disc tests,and their effective median dosage (ED50)of growth and ecdysis inhibition were 1.36mg/g and 0.34mg/g,respectively in quantitative feeding tests.For the 3-4th instar larvae of P.xylostella which are very resistant to the conventional insect-cides,the chloroform fraction of the crodc extract were very effective and its AFC50was 0.23% ,and its EC60 were 0.29% by feeding and C.22% by topical application.The servival adults were inhibited in oviposition.This study indicates that the main insecticidal priciples of the extracts exist in the weak pole parts.

       

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