Abstract: This trial was proceeded in the experimental dairy farm at South China Agricultural University in 1991 and the primary coefficient of female control was successfully reached to 81.8%at an advanced level between the internal and outside the country.As a result of the trial,11 calves were born by 10 cows,including a twin of sexuallity at birth.It is of immense values to biological science and economy for this trial.It was found that it is possible for realizing of sexing sperm by treating of bovine semen with H-y antiserum IgG.It was also an evidence of using Density Gradient Centrifugation of Sucrose solution and Fluorescent Staining for inspectation of the effectiveness of H-Y antiserum IgG.We observed that the H-Y antiserum IgG showed marked effect of inhibition on Y-bearing sperm,so that we can achieve a good result of sexing control in dairy cattle.