Abstract: Four types of explants of Panax (?) were used,in order to study induction of callus,differentiation of plantlets,hardening of regenerated plantlets and formation of roots.Experimental appproach to find a suitablepathway of differentiation,has been referred to the method for producing large numbers of plants.The results showed that explants sterilized with both HgCl2 and NaOCl had low contamination rate.Culturedon MS based nutrient medium,young inflorecencea were the best explants,which had highest frequency of callus formation and plant differentiation.In MS supplemented with 2,4-D medium,calli could directly initiate a large number of embryolds under dark condition and could initiate adventitious buds under lighting.Plant regeneration of embryoids and adventitious buds could be achieved by use of OA3 in the regeneration medium.Root formation was induced with MET.Complete plants were developed.Appeoximately 2 214 plants have been obtained.The plantlets were first successfully developed by in vetro method in 1989,From 1990 to 1991,a series of technique of obtaining high propagation were founded.