华南黄牛血液蛋白多态性及其应用研究

    A STUDY ON THE BLOOD PROTEIN POLYMORPHISM OF YELLOW CATTLE IN SOUTH CHINA AND ITS APPLICATION

    • 摘要: 本试验采用淀粉凝胶、醋酸纤维薄膜和聚丙烯酰肢凝胶电泳法测定了海南牛、雷州牛和隆林牛的血红蛋白、血清白蛋白、血液碱性磷酸酶,血清转铁蛋白、血清后转铁蛋白和血清后白蛋白等6个多态位点的基因型和基因频率。为分析华南黄牛在中国黄牛中的地位,利用有关资料,计算了我国10种黄牛间的遗传距离;用类平均法对我国的主要黄牛品种进行聚类分析,绘出10个品种的树状聚类图;用主分量分析方法绘出各品种的三维空间分布图。结果表明,华南黄牛明显与北方及中原黄牛不同,华南地区黄牛是华南地区固有的品种,由当地驯化而来,形成过程渗入其他黄牛血液的结果。

       

      Abstract: The starch gel, cellulose acetate membrane and polyacrylamide gei electrophoresis were adopted to determine the six blood protein polymorphism loci of Hainan, Leizhou and Longjin yellow cattle reported in this paper, from which the phenotypes and gene frequencies of Hb, Mb, Pa, Tf, Ptf and Akp in each breed were obtained. Standard genetic distances between three breeds and ether yeljow cattle in our country were calculated. The three dimensional space distributions were drawn by principal coordinate analysis (PCA) . The results showed that the yellow cattle in South China was different from that in North and Central China. In addition , the authors believed that the yellow cattle in South China originated from native Bos primigenius and Bos indicus with infiltration from some other yellow cattle breed blood.

       

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