Abstract: Using six varieties of indica rice as experimental materials, the study results indicated that sorbitol added to Ls medium was able to promote the growing of callus and improve its differentiation in serial subcultures. For different varieties of indica rice the growing capacity of callus and plant regeneration displayed evident varietal specificities in later stages of subcultures. After adding phytohormone with different concentrations into the differentiating medium, there were obvious disparities in differentiation effects on the organ indicated.Synchronized growing of root and bud was favoured when-kinetin (KT)and naphthalene acetic acid (NAA) were present in the ratio of 5 : 1.