微小泰泽球虫小配子发育的超微结构

    ULTRASTRUCTURAL ASPECTS OF MICROGAMETOGENESIS IN Tyzzeria parvula

    • 摘要: 采用纯种微小泰泽球虫卵囊,人工感染四日龄雏鹅,定时剖杀,取小肠组织进行超薄切片,在透射电镜下,观察微小泰泽球虫小配子体与小配子的超微结构和发育过程。小配子体由第二代裂殖子发育而来。当小配子体完成最后一次核分裂后,其核中染色质呈斑块状,核内无核仁。随后,细胞核移向小配子体边缘,并拉长成二部分,电子致密部分将发育成小配子的核,电子密度浅的另一部分则留在残体中。每个细胞核上方有一对中心粒,中心粒将进一步发育成为小配子两根鞭毛的基粒,鞭毛由此产生。成熟的小配子拥有两根鞭毛和一个体部,体部有一个长形的细胞核,核的前面嵌有一线粒体。

       

      Abstract: The ultrastructural changes occurring during microgametogony in Tyzzerio parvula were studied in tissue from the small intestine of infected young domestic geese. The development of the trophozoite into the microgametocyte was accompanied by cytoplasmic growth and nuclear division. The developing microgametocyte could be differentiated from developing schizont because the nuclei of the microgametocyte were smaller than those of the schizont and lacked nucleoli. Every nucleus migrated to the edge of the microgametocyte accompanied by the aggregating of chromatin at the periphery of the nuclei. Then each nucleus became elongated, protruding outwards from the microgametocytic membrane, and developed a dense osmiophilic area which contained the condensed chromatin, and a pale area. The dense portion of the nucleus, accompanied by the mitochondrion, entered the protrusion to become the nucleus of the microgamete, and the pale portion remained in the residual body. The two centrioles above the ucleus transformed into the basal bodies from which the flagella arose. The microgamete consisted of two flagella, an elongated nucleus, a mitochondrion located at the anterior end, and microtubules alongsaide.

       

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