Abstract: In this paper we report the effects of sodium chloride on the activity of glycolate oxidase in wheat seedlings. At 20 hrs after spraying sodium chloride on wheat seedlings, the activity of glycolate oxidase increased by 21% and the content of Protein increased by 24.2%.After the seedlings had been cultivated with 0.1, 0.2, 0.5 mol/L of sodium chloride, the glycolate oxidase activity increased by 23.0, 61.1, and 28.6 percent respectively over the control| the contents of proteinincreased by 12.8, 29.4, and 18.1 percent respectively over the control, but the chlorophyll content decreased by 2.8, 2.8, and 22.2 percent respectively. The mechanism of increase of glycolate oxidase was discussed.