Abstract: A series of experiments and otservations were dore on bile derived from different anim-species.The main physiological effects of the biles were alowing-down of the heart-rsate,inc teasing the treathing irequency and depressing blood pressure.The grass carp bile had thn strongest effects described above among the tested biles,and could disturb the bloocrcpae n lation,damage the blood vessels and cause degeneration of parenchymatous cells in the experi-mental animals.Blindnes could be caused by cropping 30% grass carp bile into the eyes.Thei results showed that the components and effeets of animal bile derived from varlous specie are different,and sometimes the differences are very great It was suggested that ore shorpl be very cautions when useing bile medicinally.