花叶病大蒜的茎端组织脱毒培养

PRELIMINARY EXPERIMENT ON VIRUS-FREE GARLIC TISSUE CULTURE

摘要: 本文论述了用MS(1962)培养基添加吲哚乙酸(IAA)、细胞激动素(KT)、萘乙酸(NAA)、2,4—二氯苯氧乙酸(2,4—D)、6—苄基嘌呤(BA)等生长调节物质,对开平金山火蒜及广州硬尾骨蒜两品种茎端0.100～0.125mm长的分生组织进行培养,获得了无毒种蒜,并提出了适于此两种大蒜无毒苗培养的培养基配方、生长调节质物的组合和浓度、以及光温条件等一套方案;论证了2,4—D、NAA、BA、光照和温度对愈伤组织诱导、芽分化和球茎形成上的作用,并对大蒜不同组织部位的培养作了初步的探讨。

Abstract: Two virus-free garlic cultivar bulbs were obtained after culturing 0.100 -0.125mm long shoot apices on the basal medium of Murashige and Skoog(1962) supplemented with different combinations and different concentration of IAA, 2,4-D, KT. NAA and BA. The effects of these growth regulators and the influences of light and temperature to the callus growth, organogenesis and bulb formtion had been compared and discussed, a set of culturing conditions and media with growth regulators were recommended.