肝脏RNA-seq揭示杜洛克猪剩余采食量的社会遗传效应调控的关键基因和分子途径

    Liver RNA-seq reveals key genes and molecular pathways regulating the social genetic effects of residual feed intake in Duroc pigs

    • 摘要:
      目的 旨在解析杜洛克猪剩余采食量的社会遗传效应(The social genetic effects of residual feed intake, SGE-RFI)的分子调控机制,揭示其通过能量代谢途径影响群体行为的生物学基础,为提高猪只饲料利用效率提供理论依据。
      方法 通过RNA测序技术对杜洛克猪肝脏组织进行全转录组分析,系统筛选与SGE-RFI性状相关的关键差异表达基因,并采用功能富集方法解析其参与的生物学通路。
      结果 鉴定出360个显著差异表达基因,功能分析表明这些基因主要富集于线粒体氧化磷酸化和ATP代谢等能量代谢相关通路,提示其可能通过调控机体能量稳态间接影响社会行为。载脂蛋白基因簇APOA1APOC3APOA4的协同表达改变,揭示了社会遗传效应调控过程中神经内分泌系统与脂代谢的交互作用。
      结论 本研究构建了杜洛克猪SGE-RFI的多维度调控网络,不仅为解析群体行为遗传机制提供了新视角,更为精准选育低剩余采食量种猪和优化群体饲养管理方案奠定了分子基础。

       

      Abstract:
      Objective This study aims to elucidate the molecular regulatory mechanisms of the social genetic effects of residual feed intake (SGE-RFI) in Duroc pigs, and reveal the biological basis of how it influences group behavior through energy metabolism pathways, thereby providing a theoretical basis for improving feed utilization efficiency in pigs.
      Method By employing RNA sequencing technology, a comprehensive transcriptome analysis of the liver tissue in Duroc pigs was conducted, systematically screening for key differentially expressed genes related to the SGE-RFI trait. Functional enrichment methods were used to analyze the biological pathways involved.
      Result The study identified 360 significantly differentially expressed genes. Functional analysis indicated that these genes were primarily enriched in energy-related pathways such as mitochondrial oxidative phosphorylation and ATP metabolism, suggesting an indirect influence on social behavior through the regulation of energy homeostasis. The coordinated expression changes in the apolipoprotein gene cluster APOA1, APOC3 and APOA4 revealed the interaction between the neuroendocrine system and lipid metabolism during the social genetic effect regulation.
      Conclusion This research has constructed a multi-dimensional regulatory network for SGE-RFI in Duroc pigs, offering not only a new perspective for understanding the genetic mechanisms of group behavior, but also laying a molecular foundation for the precise selection of low residual feed intake breeding pigs and the optimization of group feeding management strategies.

       

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