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    杨立, 蒙庆米, 白天泉, 等. 海拔对稻田金背鲤肠道结构、消化酶活性和肠道菌群的影响[J]. 华南农业大学学报, 2024, 45(6): 1-10. DOI: 10.7671/j.issn.1001-411X.202406044
    引用本文: 杨立, 蒙庆米, 白天泉, 等. 海拔对稻田金背鲤肠道结构、消化酶活性和肠道菌群的影响[J]. 华南农业大学学报, 2024, 45(6): 1-10. DOI: 10.7671/j.issn.1001-411X.202406044
    shu
    YANG Li, MENG Qingmi, BAI Tianquan, et al. Effects of altitude on gut structure, digestive enzyme activity and gut microbiota of Cyprinus carpio var. Jinbei in rice field[J]. Journal of South China Agricultural University, 2024, 45(6): 1-10. DOI: 10.7671/j.issn.1001-411X.202406044
    Citation: YANG Li, MENG Qingmi, BAI Tianquan, et al. Effects of altitude on gut structure, digestive enzyme activity and gut microbiota of Cyprinus carpio var. Jinbei in rice field[J]. Journal of South China Agricultural University, 2024, 45(6): 1-10. DOI: 10.7671/j.issn.1001-411X.202406044
    shu

    海拔对稻田金背鲤肠道结构、消化酶活性和肠道菌群的影响

    Effects of altitude on gut structure, digestive enzyme activity and gut microbiota of Cyprinus carpio var. Jinbei in rice field

      摘要
    • 摘要:
      目的 探究在不同海拔下稻田金背鲤Cyprinus carpio var. Jinbei的肠道结构、消化酶活性和肠道菌群特征。
      方法 以低(580 m)、中(830 m)和高(1340 m)3个不同海拔的稻田金背鲤为研究对象，采用组织学、酶学以及分子生物学等方法比较分析其肠道的组织结构、消化酶活性和肠道菌群特征。
      结果 低海拔组金背鲤肠道的绒毛高度(521.04 μm)显著高于中海拔组(438.01 μm)和高海拔组(419.39 μm)(P < 0.05)；中海拔组金背鲤肠道的胰蛋白酶活性(4541.65 U/mg)显著高于低海拔组(4023.72 U/mg)和高海拔组(3722.1 U/mg)(P < 0.05)；放线菌门、厚壁菌门、变形菌门、绿弯菌门和软壁菌门是稻田金背鲤肠道的主要菌群门类。
      结论 不同海拔对稻田金背鲤的肠道形态组织结构、消化酶活性和肠道菌群产生影响。虽然稻田金背鲤的肠道菌群在不同海拔下出现相应的变化，但是，仍保持以放线菌门为主的相对稳定菌群，说明金背鲤的肠道具有对稻田生态环境的适应性。

       

      Abstract:
      Objective In order to explore the gut structure, digestive enzyme activity and gut microbiota characteristics of Cyprinus carpio var. Jinbei at different altitudes.
      Method The C. carpio var. Jinbei in rice fields at low (580 m), middle (830 m) and high (1340 m) altitudes were taken as the research objects. The histology, enzymology and molecular biology methods were used to compare and analyze the gut tissue structure, digestive enzyme activity and gut microbiota characteristics.
      Result The villus height of C. carpio var. Jinbei in low altitude group (521.04 μm) was significantly higher than those of the middle altitude group (438.01 μm) and high altitude group (419.39 μm) (P< 0.05). The trypsin activity of C. carpio var. Jinbei in the middle altitude group (4541.65 U/mg) was significantly higher than those of the low altitude group (4023.72 U/mg) and the high altitude group (3722.1 U/mg) (P<0.05). Actinobacteria, Firmicutes, Proteobacteria, Chloroflexi and Tenericutes were the main gut microbiota of C. carpio var. Jinbei in rice field.
      Conclusion Different altitudes affect the intestinal morphology, digestive enzyme activity, and gut microbiota structure of the C. carpio var. Jinbei in rice field. Although the gut microbiota of C. carpio var. Jinbei in rice field changes at different altitudes, it still maintained a relatively stable gut microbiota dominated by Actinobacteria, indicating that the gut of C. carpio var. Jinbei has been adapted to the ecological environment of rice field.

       

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