大花紫薇EMS诱变育种研究初探

    Study on EMS Mutagenesis Breeding of Lagerstroemia speciosa

    • 摘要:
      目的 为了丰富大花紫薇育种方式,探究EMS诱变其最适条件。
      方法 以大花紫薇的成熟种子为研究材料,比较和分析EMS不同浓度和处理时间对大花紫薇种子萌发及幼苗生长的影响。
      结果 EMS处理能够抑制大花紫薇种子萌发,浓度越高、处理时间越长,抑制作用越明显,20g·L−1EMS处理12 h后全部致死;随着EMS浓度变高、处理时间变长,种子发芽起始时间推迟,发芽持续时间变长胚轴及胚根生长受到的抑制越显著;16g·L−1EMS处理12 h和18 g·L−1EMS处理10 h,其相对致死率为47.99%和55.02%,最接近半致死剂量,为大花紫薇EMS诱变可选择的处理组合;EMS处理抑制大花紫薇幼苗生长,且在20g·L−1EMS处理8 h时最明显,其株高、冠幅、地径、叶面积分别为8.28cm、9.66cm、1.70mm和29.32 cm2,而叶形指数在16g·L−1EMS处理12 h和18 g·L−1EMS处理10 h最大,为2.42。
      结论 本研究确定了接近大花紫薇种子EMS诱变半致死剂量的处理组合,探究EMS浓度和时间处理对大花紫薇种子萌发和幼苗生长的影响,可为该植物的新品种培育提供基础。

       

      Abstract:
      Objective In order to enrich the breeding methods of Lagerstroemia speciosa, the optimum conditions of EMS mutagenesis were studied..
      Method The seeds of Lagerstroemia speciosa were used as the study material to compare and analyse the effects of different combination of EMS concentrations and treatment times on seed germination and seedling growth ofLagerstroemia speciosa.
      Result It was observed that EMS treatment could inhibit the germination of Lagerstroemia speciosa seeds. The concentration and treatment time were found to have a significant impact on the degree of inhibition. All seeds were killed by treating with 20g·L−1EMSfor 12 h. The starting time of seed germination was delayed and the germination duration became longer with the increase of EMS concentration and treatment time. The inhibition of hypocotyl and radicle growth was more significant. The relative lethal rates of the 16g·L−1 EMS treatment for 12 h and the 18g·L−1 EMS treatment for 10 h were 47.99% and 55.02%, respectively, which were the closest to the Lethal concentration 50(LC50). Consequently, the 1.6% EMS treatment for 12 h and the 18g·L−1 EMS treatment for 10 hours were identified as the optimal treatment combinations for EMS mutagenesis of Lagerstroemia speciosa. The growth of Lagerstroemia speciosa seedlings was inhibited by EMS treatment, with the greatest impact observed at 20g·L−1 EMS treatment for 8 h. The plant height, crown width, ground diameter and leaf area were 8.28 cm, 9.66 cm, 1.70 mm and 29.32 cm2. The leaf shape index was found to be the highest at 16g·L−1 EMS treatment for 12 h and 18g·L−1 EMS treatment for 10 h, with a value of 2.42.
      Conclusion In this study, the treatment combination close to the LC50 of EMS mutagenesis of Lagerstroemia speciosa seeds was determined, and the effects of EMS concentration and treatment time on seed germination and seedling growth of Lagerstroemia speciosa were explored, which could provide a basis for the cultivation of new varieties of this plant.

       

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