Abstract:
Objective To realize early detection, prevention and control of Salmonella enteritidis, a detection method was established by combining CRISPR(Clustered regularly interspaced short palindromic repeat) technology with polymerase chain reaction (PCR).
Method PCR primers were screened based on the conserved sdfI gene sequence. The sensitivity test was conducted using S. enteritidis genomes at different concentrations, while the specificity tests were conducted using the genomes of Escherichia coli, Staphylococcus aureus, Pasteurella multocida, Salmonella choleraesuis and Enterococcus faecalis. Subsequently, the developed method was applied for the detection of S. enteritidis in small intestinal samples.
Result This method was capable of identifying S. enteritidis in small intestinal samples, with a detection limit of 1.72 × 100 μL−1, showing excellent specificity and no cross-reactivity with E. coli, S. aureus, P. multocida, S. choleraesuis and E. faecalis.
Conclusion This study develops a compact diagnostic method for the early detection of S. enteritidis, showing excellent sensitivity and specificity. This innovation presents a fresh perspective for the swift identification of S. enteritidis.