薇甘菊提取物对稻瘟病菌的抑菌活性

杨态娇; 张威; 罗建梅; 李青; 聂闪闪; 廖仕梅; 符滔; 闫晓慧

doi:10.7671/j.issn.1001-411X.202311015

薇甘菊提取物对稻瘟病菌的抑菌活性

Antifungal activity of Mikania micrantha against Magnaporthe oxyzae

摘要

摘要:
目的以入侵物种薇甘菊Mikania micrantha为材料，探究其不同提取物及组分对稻瘟病菌Magnaporthe oxyzae的抑菌活性。
方法以稻瘟病菌为供试病原菌，采用生长速率法对采自云南德宏的薇甘菊植物提取物进行室内抑菌活性测定，并通过柱层析对提取物的抑菌活性组分进行追踪。
结果在初筛浓度为1 mg/mL时，薇甘菊乙酸乙酯萃取物对稻瘟病菌有较好抑菌活性，抑菌率为49.84%。对薇甘菊乙酸乙酯萃取物的14个柱层析组分进行抑菌活性追踪，组分Fr₅、Fr₆、Fr₁₂、Fr₁₃抑菌效果显著，EC₅₀分别为1.856、1.846、0.634、0.29 mg/mL；4个组分均使菌丝质量减轻、MDA含量升高、菌丝形态畸变、孢子数量减少。
结论本研究发现薇甘菊对稻瘟病菌有较好的抑菌效果，为综合开发利用薇甘菊提供了新思路，也为稻瘟病菌的绿色防控提供了科学依据。

Abstract:
Objective Mikania micrantha, an invasive species, was used as a material to investigate the antifungal activity of its different extracts and components against Magnaporthe oxyzae.

Method The antifungal activity of extract of M. micrantha from Dehong, Yunnan Province was determined by growth rate method with M. oxyzae as the test pathogen, and the antifungal active components of the extract were traced by column chromatography.
Result At the initial screening concentration of 1 mg/mL, M. micrantha ethylacetate extract showed potent antifungal activity against M. oxyzae, with an inhibition rate of 49.84%. The antifungal activity tracking of 14 column chromatography fractions of the ethylacetate extract showed that the antifungal activity of Fr₅, Fr₆, Fr₁₂ and Fr₁₃ was significant, EC₅₀ values were 1.856, 1.846, 0.634 and 0.29 mg/mL, respectively. All the four active components resulted in the reduction of mycelial mass, elevation of MDA content, aberration of mycelial morphology, and reduction of spore number of M. oxyzae.
Conclusion This study finds that M. micrantha can significantly inhibit M. oxyzae, which provides a new idea for comprehensive development and utilization of M. micrantha, and also provides a scientific basis for green control of M. oxyzae.

HTML全文

参考文献(26)

施引文献

资源附件(0)