丁氏稻种资源有利基因挖掘与创新研究进展

陈志雄; 王兰; 吴锦文; 刘向东

doi:10.7671/j.issn.1001-411X.202307016

丁氏稻种资源有利基因挖掘与创新研究进展

广东省植物分子育种重点实验室/亚热带农业生物资源保护与利用国家重点实验室/岭南现代农业科学与技术广东省实验室/华南农业大学农学院, 广东广州 510642

基金项目: 岭南水稻种质资源基地库项目(2023-40)；岭南现代农业实验室科研项目(NT2021001)

详细信息

作者简介:
陈志雄，副研究员，博士，主要从事稻种资源保护与创新研究，E-mail: chenzx@scau.edu.cn

通讯作者:
刘向东，教授，博士，主要从事稻种资源保护与创新(多倍体水稻遗传育种)研究，E-mail: xdliu@scau.edu.cn

中图分类号: S511；S334
计量
- 文章访问数: 114
- HTML全文浏览量: 23
- PDF下载量: 32
出版历程
- 收稿日期: 2023-07-29
- 网络出版日期: 2023-11-12
- 发布日期: 2023-09-06
- 刊出日期: 2023-09-09

Research progress in favorable gene mining and innovation of Ting’s rice germplasm

Guangdong Provincial Key Laboratory of Plant Molecular Breeding/State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Guangdong Laboratory for Lingnan Modern Agriculture/College of Agriculture, South China Agricultural University, Guangzhou 510642, China

摘要

摘要:
稻种资源是水稻生物育种的重要物质基础。我国保存的稻种资源数量超过9万份，丁氏稻种资源(Ting’s rice germplasm)是极具特色的一类，主要包括丁颖在20世纪20—30年代广泛收集的7 000多份各地的栽培稻、卢永根在20世纪90年代组织收集的2 000份野生稻资源以及所创制的新型四倍体水稻新种质等。本文总结了近20多年来丁氏稻种资源的研究进展，并提出了今后研究应重点解决的问题，为水稻育种更好地利用该资源提供参考。
- 水稻 /
- 稻种资源 /
- 育性 /
- 生物育种
Abstract:
Rice germplasm provides an important fundation for biological breeding. The total number of rice germplasm preserved in China exceeds 90000, among which Ding’s rice germplasm is a unique category. The Ding’s rice germplasm mainly included over 7000 cultivated rice varieties collected by DING Ying in various regions from 1920s to 1930s, 2 000 wild rice lines collected by LU Yonggen in the 1990s, the newly-devolped neo-tetraploid rice germplasm, and so on. This article summarized the research progress of Ding’s rice germplasm in the past 20 years and proposed the key subjects in future research, with the aim of providing a reference for better utilization of this germplasm in rice breeding.
- Oryza sativa L. /
- Rice germplasm /
- Fertility /
- Biological breeding

HTML全文

表 1 从丁氏收集稻种资源中鉴定的潜在优异基因资源

Table 1 Potential and superior gene resources identified from Ting’s rice germplasm collection

基因/QTL¹⁾ Gene/QTL	染色体 Chromosome	耐逆性 Stress resistance	基因/QTL¹⁾ Gene/QTL	染色体 Chromosome	耐逆性 Stress resistance	基因/QTL¹⁾ Gene/QTL	染色体 Chromosome	耐逆性 Stress resistance
QTL1~QTL3^a	Chr1	冷害	Os01g57350^c	Chr1	铝毒	qRE7^d	Chr11	锌毒
QTL4~QTL13^a	Chr2	冷害	Os01g57360^c	Chr1	铝毒	qRRE1~qRRE2^d	Chr1	锌毒
QTL14~QTL21^a	Chr3	冷害	Os01g74200^c	Chr1	铝毒	qRRE4^d	Chr2	锌毒
QTL22^a	Chr5	冷害	Os03g30060^c	Chr3	铝毒	qRRE4~qRRE6^d	Chr3	锌毒
qALT1.1~qALT1.6^b	Chr1	铝毒	Os03g30060^c	Chr3	铝毒	qRRE7^d	Chr4	锌毒
qALT2.1~qALT2.3^b	Chr2	铝毒	Os07g03050^c	Chr7	铝毒	qRRE8~qRRE9^d	Chr6	锌毒
qALT3.1~qALT3.7^b	Chr3	铝毒	Os09g33550^c	Chr9	铝毒	qRRE10^d	Chr7	锌毒
qALT4.1~qALT4.2^b	Chr4	铝毒	Os11g03110^c	Chr11	铝毒	qRRE11~qRRE12^d	Chr8	锌毒
qALT6.1~qALT6.2^b	Chr6	铝毒	qRE1~qRE2^d	Chr2	铝毒	qRRE13^d	Chr9	锌毒
qALT7.1~qALT7.2^b	Chr7	铝毒	qRE3~qRE4^d	Chr6	铝毒	qRRE14^d	Chr10	锌毒
qALT9.1^b	Chr9	铝毒	qRE5^d	Chr8	锌毒	qRRE15~qRRE17^d	Chr11	锌毒
qALT11.1~qALT11.2^b	Chr11	铝毒	qRE6^d	Chr9	锌毒	qRRE18~qRRE19^d	Chr11	锌毒
1) a~d分别表示鉴定方法为GWAS (SLAF)^[9]、GWAS (SLAF)^[12]、GWAS (re-squencing、transcriptome)^[13]和GWAS (SLAF)^[14]；QTL5、QTL17分别与OsFAD2、OsMYB2共定位^[9]，qALT1.6与OsFRDL4、qALT2.2与Os02g49790、qALT3.1与OsApx1、qALT6.2与STAR1共定位^[12]，qRRE1、qRRE15分别与OsMTI-3a、qZRTDW11共定位^[14] 　1) a−d indicate the identification methods were GWAS (SLAF)^[9], GWAS (SLAF)^[12], GWAS (re-squencing, transcriptome)^[13] and GWAS (SLAF)^[14], respectively; QTL5 and OsFAD2^[9], QTL17 and OsMYB2^[9], qALT1.6 and OsFRDL4^[12], qALT2.2 and Os02g49790^[12], qALT3.1 and OsApx1^[12], qALT6.2 and STAR1^[12], qRRE1 and OsMTI-3a^[14], qRRE15 and qZRTDW11^[14], are co-located

下载: 导出CSV

表 2 丁氏稻种资源野生稻资源的有利基因资源

Table 2 Favorable gene resources from wild rice of Ting’s rice germplasm

基因/QTL Gene/QTL	染色体 Chromosome	功能 Function	来源 Source	鉴定方法 Identification method
OoADF1		耐盐	药用野生稻	同源克隆
S_bⁿ	Chr5	克服籼粳杂种花粉不育	广东高州普通野生稻以及IRW28	分子标记
S_dⁿ	Chr1	克服籼粳杂种花粉不育	广东高州普通野生稻以及IRW28	分子标记
S_eⁿ	Chr12	克服籼粳杂种花粉不育	广东高州普通野生稻以及IRW28	分子标记
qRRE-6-2	Chr6	耐铝	广东高州普通野生稻	QTL定位
qRRE-7-2	Chr7	耐铝	广东高州普通野生稻	QTL定位
qGL3.5	Chr3	调控粒型	广东遂溪普通野生稻	基因定位与克隆
GSW3	Chr3	调控粒型	广东遂溪普通野生稻	基因定位与克隆

下载: 导出CSV

表 3 同源四倍体水稻减数分裂期间差异表达基因

Table 3 Differently expressed genes during meiosis of autotetraploid rice

基因来源 Gene source	花粉育性/% Pollen fertility	差异基因数 No. of differently expressed genes	下调基因数 No. of down regulated genes	涉及已知基因 Involved and identified genes	参考文献 Reference
台中65-4x	73.67	786	125	PAIR2、OsDMC1B等	[51]
T449	54.09	75	60	OsMTOPVIB、OsMOF等	[53]
02428-4x	43.30	663	352	OsMYB80、OsABCG15、PTC1、CYP703A3等	[54]

下载: 导出CSV

表 4 利用高州野生稻单片段代换系检出的重要农艺性状QTLs

Table 4 QTLs of important agronomic trait detected by SSSL of wild rice in Gaozhou

代换系编号 SSSL code	QTL	性状 Trait	染色体 Chromosome	定位片段 Located segment	加性效应 Additive effect	加性效应比例/% Additive effect proportion
2	Pss-2-2	结实率	Chr2	RM530—RM208—长臂末端	−0.345	37.5
5	Pss-11-1	结实率	Chr11	RM21—PSM366—PSM417	−0.305	33.1
6	Lfl-6-1	剑叶长	Chr6	RM253—RM527—RM539—RM3—PSM138	−8.165	25.3
6	Wfl-6-1	剑叶宽	Chr6	RM253—RM527—RM539—RM3—PSM138	−7.865	24.3
9	Lfl-2-1	剑叶长	Chr2	RM110—RM211—RM279—PSM116	−0.340	16.3
9	Wfl-2-1	剑叶宽	Chr2	RM110—RM211—RM279—PSM116	−0.490	23.6

下载: 导出CSV

参考文献(71)

[1]	KHUSH G S. What it will take to feed 5.0 billion rice consumers in 2030[J]. Plant Molecular Biology, 2005, 59(1): 1-6. doi: 10.1007/s11103-005-2159-5
[2]	GODFRAY H C, BEDDINGTON J R, CRUTE I R, et al. Food security: The challenge of feeding 9 billion people[J]. Science, 2010, 327(5967): 812-818. doi: 10.1126/science.1185383
[3]	吴比, 胡伟, 邢永忠. 中国水稻遗传育种历程与展望[J]. 遗传, 2018, 40(10): 841-857. doi: 10.16288/j.yczz.18-213
[4]	李晓玲, 李金泉, 卢永根. 水稻核心种质的构建策略研究[J]. 沈阳农业大学学报, 2007, 38(5): 681-687. doi: 10.3969/j.issn.1000-1700.2007.05.007
[5]	李自超, 张洪亮, 曹永生, 等. 中国地方稻种资源初级核心种质取样策略研究[J]. 作物学报, 2003, 29(1): 20-24. doi: 10.3321/j.issn:0496-3490.2003.01.004
[6]	李晓玲. 栽培稻种质资源核心种质构建的研究[D]. 广州: 华南农业大学, 2006.
[7]	ZHANG P, LI J Q, LI X L, et al. Population structure and genetic diversity in a rice core collection (Oryza sativa L.) investigated with SSR markers[J]. PLoS One, 2011, 6(12): e27565. doi: 10.1371/journal.pone.0027565
[8]	ZHANG P, ZHONG K Z, ZHONG Z Z, et al. Genome-wide association study of important agronomic traits within a core collection of rice (Oryza sativa L. )[J]. BMC Plant Biology, 2019, 19: 259. doi: 10.1186/s12870-019-1842-7.
[9]	SONG J Y, LI J Q, SUN J, et al. Genome-wide association mapping for cold tolerance in a core collection of rice (Oryza sativa L.) landraces by using high-density single nucleotide polymorphism markers from specific-locus amplified fragment sequencing[J]. Frontiers in Plant Science, 2018, 9: 875. doi: 10.3389/fpls.2018.00875.
[10]	FU D, ZHONG K Z, ZHONG Z Z, et al. Genome-wide association study of sheath blight resistance within a core collection of rice (Oryza sativa L. )[J]. Agronomy, 2022, 12(7): 1493. doi: 10.3390/agronomy12071493.
[11]	ZHANG P, ZHONG K Z, TONG H H, et al. Association mapping for aluminum tolerance in a core collection of rice landraces[J]. Frontiers in Plant Science, 2016, 7: 1415. doi: 10.3389/fpls.2016.01415.
[12]	ZHAO M H, SONG J Y, WU A T, et al. Mining beneficial genes for aluminum tolerance within a core collection of rice landraces through genome-wide association mapping with high density SNPs from specific-locus amplifified fragment sequencing[J]. Frontier in Plant Science, 2018, 9: 1838. doi: 10.3389/fpls.2018.01838.
[13]	ZHANG P, ZHONG K Z, ZHONG Z Z, et al. Mining candidate gene for rice aluminum tolerance through genome wide association study and transcriptomic analysis[J]. BMC Plant Biology, 2019, 19(1): 490. doi: 10.1186/s12870-019-2036-z.
[14]	ZHONG K Z, XIE L H, HU S K, et al. Genome-wide association study of zinc toxicity tolerance within a rice core collection (Oryza sativa L. )[J]. Plants, 2022, 11(22): 3138. doi: 10.3390/plants11223138.
[15]	SHAHID M Q, CHEN F Y, LI H Y, et al. Double-neutral genes, Sa-n and Sb-n, for pollen fertility in rice to overcome indica × japonica hybrid sterility[J]. Crop Science, 2013, 53(1): 164-176. doi: 10.2135/cropsci2012.07.0451
[16]	杨有新, 吴锦文, 陈志雄, 等. 基于功能性标记和测序发掘携带有S₅ⁿ基因的水稻新种质[J]. 科学通报, 2009, 54(15): 2212-2218.
[17]	李宏岩, 王思哲, SHAHID M Q, 等. 从携带S₅ⁿ基因的水稻种质中发掘S_aⁿ、S_bⁿ和S_cⁿ基因[J]. 作物学报, 2013, 39(8): 1366-1376.
[18]	张欢欢, 刘蕊, 郭海滨, 等. 药用野生稻有利基因发掘与利用研究进展[J]. 中国农学通报, 2009, 25(19): 42-45.
[19]	潘小芬. 药用野生稻TAC文库的构建[D]. 广州: 华南农业大学, 2006.
[20]	汪暖, 陈志雄, 刘蕊, 等. 药用野生稻TAC克隆转化籼稻的体系初探[J]. 植物生理学通讯, 2010, 46(3): 217-222.
[21]	刘蕊, 张欢欢, 陈志雄, 等. 筛选和转化药用野生稻TAC克隆获得耐旱水稻[J]. 中国农业科学, 2014, 47(8): 1445-1457. doi: 10.3864/j.issn.0578-1752.2014.08.001
[22]	LIU R, ZHANG H H, CHEN Z X, et al. Drought-tolerant rice germplasm developed from an Oryza officinalis transformation-competent artificial chromosome clone[J]. Genetics and Molecular Research, 2015, 14(4): 13667-13678. doi: 10.4238/2015.October.28.29
[23]	李培纲, 李红梅, 张帅, 等. 药用野生稻ADF基因的克隆及其抗逆性分析[J]. 华北农学报, 2017, 32(6): 37-44. doi: 10.7668/hbnxb.2017.06.006
[24]	卢永根, 刘向东, 陈雄辉. 广东高州普通野生稻的研究进展[J]. 植物遗传资源学报, 2008, 9(1): 1-5. doi: 10.13430/j.cnki.jpgr.2008.01.012
[25]	王兰, 蔡东长. 高州普通野生稻苗期耐寒性鉴定及其SSR多态标记分析[J]. 华北农学报, 2011, 26(6): 12-15. doi: 10.7668/hbnxb.2011.06.003
[26]	LIU W, GHOURI F, YU H, et al. Genome wide re-sequencing of newly developed rice lines from common wild rice (Oryza rufipogon Griff. ) for the identification of NBS-LRR genes[J]. PLoS One, 2017, 12(7): e0180662. doi: 10.1371/journal.pone.0180662
[27]	YU H, SHAHID M Q, LI R B, et al. Genome-wide analysis of genetic variations and the detection of rich variants of NBS-LRR encoding genes in common wild rice lines[J]. Plant Molecular Reporter, 2018, 36(4): 618-630. doi: 10.1007/s11105-018-1103-1
[28]	CHEN J J, DING J H, OUYANG Y D, et al. A triallelic system of S₅ is a major regulator of the reproductive barrier and compatibility of indica-japonica hybrids in rice[J]. Proceedings of the National Academy of Sciences of the United States of America, 2008, 105(32): 11436-11441.
[29]	魏常敏, 王兰, 杨有新, 等. 普通野生稻中S₅ⁿ基因的鉴定及其胚囊育性研究[J]. 科学通报, 2010, 55(11): 1007-1014.
[30]	TONG J F, LI Y H, YANG Y X, et al. Molecular evolution of rice S₅ⁿ and functional comparison among different sequences[J]. Grop Germplasm Resources, 2011, 56(19): 2016-2024.
[31]	PENG H, SHAHID M Q, LI Y H, et al. Molecular evolution of S₅ locus and large differences in its coding region revealed insignificant effect on indica × japonica embryo sac fertility[J]. Plant Systematics and Evolution, 2015, 301(2): 639-655. doi: 10.1007/s00606-014-1102-0
[32]	史磊刚, 刘向东, 刘博, 等. 从普通野生稻中鉴定栽培稻F₁花粉不育座位S_b的中性基因[J]. 科学通报, 2009, 54(19): 2967-2974.
[33]	LIU B, LI J Q, SHAHID M Q, et al. Identification of neutral genes at pollen sterility loci S_d and S_e of cultivated rice (Oryza sativa) with wild rice (O. rufipogon) origin[J]. Genetics and Molecular Research, 2011, 10(4): 3435-3445. doi: 10.4238/2011.October.31.10
[34]	LI J Q, SHAHID M Q, FENG J H, et al. Identification of neutral alleles at pollen sterility gene loci cultivated rice (Oryza sativa L.) from wild rice (O. rufipogon Griff.)[J]. Plant Systematics and Evolution, 2012, 298(1): 33-42. doi: 10.1007/s00606-011-0520-5
[35]	杨培周, 郭海滨, 赵杏娟, 等. 广东高州普通野生稻生殖特性的研究[J]. 植物遗传资源学报, 2006, 7(2): 136-143. doi: 10.3969/j.issn.1672-1810.2006.02.002
[36]	练子贤, 魏常敏, 卢永根, 等. 广东高州普通野生稻与粳稻杂交F₁胚囊育性及发育特点[J]. 中国水稻科学, 2008, 22(3): 266-272. doi: 10.3321/j.issn:1001-7216.2008.03.008
[37]	ZHANG L S, SHIVUTE F N, SHAHID M Q, et al. In vitro induction of auto-allotetraploid in a newly developed wild rice line from Oryza alta Swallen[J]. Plant Cell, Tissue and Organ Culture, 2019, 139(3): 577-587. doi: 10.1007/s11240-019-01701-8
[38]	FU X L, LU Y G, LIU X D, et al. Cytological mechanisms of interspecific incrossability and hybrid sterility between Oryza sativa L. and O. alta Swallen[J]. Chinese Science Bulletin, 2007, 52(6): 755-765. doi: 10.1007/s11434-007-0138-8
[39]	傅雪琳, 刘向东, 卢永根. 亚洲栽培稻与短花药野生稻种间杂交障碍观察[J]. 华南农业大学学报, 2013, 34(3): 287-291. doi: 10.7671/j.issn.1001-411X.2013.03.002
[40]	FU X L, LU Y G, LIU X D, et al. Cytological behavior of hybridization barriers between Oryza sativa and Oryza officinalis[J]. Agricultural Sciences in China, 2011, 10(10): 1489-1500. doi: 10.1016/S1671-2927(11)60143-0
[41]	褚绍尉, 王林, 刘桂富, 等. 广东高州普通野生稻耐铝性及其QTL定位[J]. 华北农学报, 2013, 28(3): 12-18. doi: 10.3969/j.issn.1000-7091.2013.03.003
[42]	王兰, 李智, 郑杏梅, 等. 普通野生稻矮化突变体的株高与分蘖基因的QTL定位及主效基因的遗传分析[J]. 华北农学报, 2014, 29(5): 5-9. doi: 10.7668/hbnxb.2014.05.002
[43]	郑跃滨, 李智, 赵海燕, 等. 水稻粒长QTL定位与主效基因的遗传分析[J]. 西北植物学报, 2020, 40(4): 598-604. doi: 10.7606/j.issn.1000-4025.2020.04.0598
[44]	WANG L, LIU Y, ZHAO H Y, et al. Identification of qGL3.5, a novel locus controlling grain length in rice through bulked segregant analysis and fine mapping[J]. Frontiers in Plant Science, 2022, 13: 921029. doi: 10.3389/fpls.2022.921029.
[45]	BAI F, MA H J, CAI Y C, et al. Natural allelic variation in GRAIN SIZE AND WEIGHT 3 of wild rice regulates the grain size and weight[J/OL]. Plant Physiology, (2023-06-24) [2023-07-30]. 2023. doi: 10.1093/plphys/kiad320.
[46]	张华华, 冯九焕, 卢永根, 等. 利用激光扫描共聚焦显微镜观察同源四倍体水稻胚囊的形成与发育[J]. 电子显微学报, 2003, 22(5): 380-384. doi: 10.3969/j.issn.1000-6281.2003.05.006
[47]	王兰, 刘向东, 卢永根, 等. 同源四倍体水稻胚乳发育: 极核融合和胚乳细胞化[J]. 中国水稻科学, 2004, 18(4): 281-289. doi: 10.3321/j.issn:1001-7216.2004.04.001
[48]	王兰, 刘向东, 卢永根, 等. 同源四倍体水稻胚乳发育: 糊粉层乳淀粉积累及胼胝质“套”的形成[J]. 中国水稻科学, 2004, 18(6): 507-514. doi: 10.3321/j.issn:1001-7216.2004.06.007
[49]	郭海滨, 卢永根, 冯九焕, 等. 利用激光扫描共聚焦显微术对同源四倍体水稻胚囊形成与发育的进一步观察[J]. 激光生物学报, 2006, 15(2): 111-117. doi: 10.3969/j.issn.1007-7146.2006.02.001
[50]	HE J H, SHAHID M Q, LI Y J, et al. Allelic interaction of F₁ pollen sterility loci and abnormal chromosome behaviour caused pollen sterility in inter-subspecific autotetraploid rice hybrids[J]. Journal of Experimental Botany, 2011, 62(13): 4433-4445. doi: 10.1093/jxb/err098
[51]	WU J W, SHAHID M Q, GUO H B, et al. Comparative cytological and transcriptomic analysis of pollen development in autotetraploid and diploid rice[J]. Plant Reproduction, 2014, 27(4): 181-196. doi: 10.1007/s00497-014-0250-2
[52]	HU C Y, ZENG Y X, LU Y G, et al. High embryo sac fertility and diversity of abnormal embryo sacs detected in autotetraploid indica/japonica hybrids in rice by whole-mount eosin B-staining confocal laser scanning microscopy[J]. Plant Breeding, 2009, 128(2): 187-192. doi: 10.1111/j.1439-0523.2008.01555.x
[53]	CHEN L, SHAHID M Q, WU J, et al. Cytological and transcriptome analyses reveal abrupt gene expression for meiosis and saccharide metabolisms that associated with pollen abortion in autotetraploid rice[J]. Molecular Genetics and Genomics, 2018, 293(6): 1407-1420. doi: 10.1007/s00438-018-1471-0
[54]	LI X, YU H, JIAO Y M, et al. Genome-wide analysis of DNA polymorphisms, the methylome and transcriptome revealed that multiple factors are associated with low pollen fertility in autotetraploid rice[J]. PLoS One, 2018, 13(8): e0201854. doi: 10.1371/journal.pone.0201854
[55]	LI X, SHAHID M Q, WU J W, et al. Comparative small RNA analysis of pollen development in autotetraploid and diploid rice[J]. International Journal of Molecular Sciences, 2016, 17(4): 499. doi: 10.3390/ijms17040499.
[56]	LI X, SHAHID M Q, XIA J, et al. Analysis of small RNAs revealed differential expressions during pollen and embryo sac development in autotetraploid rice[J]. BMC Genomics, 2017, 18: 129. doi: 10.1186/s12864-017-3526-8.
[57]	LI X, SHAHID M Q, WEN M S, et al. Global identification and analysis revealed differentially expressed lncRNAs associated with meiosis and low fertility in autotetraploid rice[J]. BMC Plant Biology, 2020, 20(1): 82. doi: 10.1186/s12870-020-2290-0.
[58]	GUO H B, MENDRIKAHY J N, XIE L, et al. Transcriptome analysis of neo-tetraploid rice reveals specific differential gene expressions associated with fertility and heterosis[J]. Scientific Reports, 2017, 7: 40139. doi: 10.1038/srep40139.
[59]	刘向东, 吴锦文, 陆紫君, 等. 同源四倍体水稻: 低育性机理、改良与育种展望[J/OL]. 遗传, (2023-06-28)[2023-07-30]. https://kns.cnki.net/kcms2/detail/11.1913.R.20230626.2143.002.html.
[60]	CHEN L, YUAN Y, WU J W, et al. Carbohydrate metabolism and fertility related genes high expression levels promote heterosis in autotetraploid rice harboring double neutral genes[J]. Rice, 2019, 12: 34. doi: 10.1186/s12284-019-0294-x.
[61]	GHALED M A A, LI C, SHAHID M Q, et al. Heterosis analysis and underlying molecular regulatory mechanism in a wide-compatible neo-tetraploid rice line with long panicles[J]. BMC Plant Biology, 2020, 20(1): 83. doi: 10.1186/s12870-020-2291-z.
[62]	张桂权. 基于SSSL文库的水稻设计育种平台[J]. 遗传, 2019, 41(8): 754-760. doi: 10.16288/j.yczz.19-105
[63]	LIN S J, LIU Z P, ZHANG K, et al. GL9 from Oryza glumaepatula controls grain size and chalkiness in rice[J]. Crop Journal, 2023, 11(1): 198-207. doi: 10.1016/j.cj.2022.06.006
[64]	ZHAO H Y, FU Y, ZHANG G Q, et al. GS6.1 controls kernel size and plant architecture in rice[J]. Planta, 2023, 258(2): 42. doi: 10.1007/s00425-023-04201-4.
[65]	ZHAN P L, MA S P, XIAO Z L, et al. Natural variations in grain length 10 (GL10) regulate rice grain size[J]. Journal of Genetics and Genomics, 2022, 49(5): 405-413. doi: 10.1016/j.jgg.2022.01.008
[66]	ZHAN P L, WEI X, XIAO Z L, et al. GW10, a member of P450 subfamily regulates grain size and grain number in rice[J]. Theoretical and Applied Genetics, 2021, 134(12): 3941-3950. doi: 10.1007/s00122-021-03939-3
[67]	PEI R Q, ZHANG Z G, HUANG M C, et al. Mapping QTLs controlling low-temperature germinability in rice by using single segment substitution lines derived from 4 AA-genome species of wild rice[J]. Euphytica, 2021, 217(4): 58. doi: 10.1007/s10681-021-02791-2.
[68]	TAN Q Y, ZOU T, ZHENG M M, et al. Substitution mapping of the major quantitative trait loci controlling stigma exsertion rate from Oryza glumaepatula[J]. Rice, 2020, 13(1): 37. doi: 10.1186/s12284-020-00397-1.
[69]	WANG S K, LI S, LIU Q, et al. The OsSPL16-GW7 regulatory module determines grain shape and simultaneously improves rice yield and grain quality[J]. Nature Genetics, 2015, 47(8): 949-954. doi: 10.1038/ng.3352
[70]	WANG S K, WU K, YUAN Q B, et al. Control of grain size, shape and quality by OsSPL16 in rice[J]. Nature Genetics, 2012, 44(8): 950-954. doi: 10.1038/ng.2327
[71]	赵杏娟. 广东高州普通野生稻单片段代换系的构建及分蘖数QTL鉴定[D]. 广州: 华南农业大学, 2008.

施引文献(19)

期刊类型引用(10)

1.	何玉，周晨莉，张恒嘉. 水氮互作对土壤有机碳、微生物及酶活性的影响研究述评. 水利规划与设计. 2025(03): 97-100+106 . 百度学术
2.	秦钧，骆洪义，贾国燏，褚旭，宋久洋，胡华林. 聚乙烯微塑料对土壤的影响及综合生物标志物响应指数. 山东化工. 2025(03): 12-18 . 百度学术
3.	赵俊波，胡兵辉. 水肥调控对茶树土壤酶及土壤养分的影响. 山西农业大学学报(自然科学版). 2024(02): 130-140 . 百度学术
4.	梁榕，何娇，孙飞虎，张瑞芳，王鑫鑫. 聚乙烯微塑料对土壤养分和酶活性的影响. 环境科学. 2024(06): 3679-3687 . 百度学术
5.	王颜玉，王文定，郑梦瑶，欧行奇，郑会芳. 施氮和灌溉处理对麦田土壤有机碳组分及酶活性的影响. 环境工程技术学报. 2024(05): 1419-1426 . 百度学术
6.	朱琪，史中兴，寇燕燕，刘斌，陈亮，栾倩倩. 原位工程化根治技术和增施生物有机肥对盐碱地土壤酶活性及甜瓜产量、品质的影响. 中国瓜菜. 2023(03): 77-84 . 百度学术
7.	赵朔. 水肥一体化模式对马铃薯干物质积累及水分利用效率的影响. 基层农技推广. 2023(05): 34-37 . 百度学术
8.	郝海波，许文霞，侯振安. 水氮耦合对滴灌棉田土壤有机碳组分及酶活性的影响. 植物营养与肥料学报. 2023(05): 860-875 . 百度学术
9.	吕江艳，龙鹏宇，罗维钢，李伏生，农梦玲. 甘蔗节水高产和蔗田氧化亚氮减排的滴灌施肥模式. 节水灌溉. 2023(12): 1-8 . 百度学术
10.	关追追，卢奇锋，陈动，邱权，苏艳，李吉跃，何茜. 施肥方式对幼龄楸树非结构性碳器官分配和生长季动态的影响. 西北植物学报. 2022(08): 1355-1362 . 百度学术