水稻次生代谢物质介导红腹缢管蚜与白背飞虱的种间互作关系

胡杰; 贡常委; 袁好; 蒲建; 王学贵

doi:10.7671/j.issn.1001-411X.202305006

水稻次生代谢物质介导红腹缢管蚜与白背飞虱的种间互作关系

Interspecific relationships between Rhopalosiphum rufiabdominalis and Sogatella furcifera mediated by rice secondary metabolites

摘要

摘要:
目的探究白背飞虱Sogatella furcifera与红腹缢管蚜Rhopalosiphum rufiabdominalis种间互作机制，以及2种昆虫互作过程中水稻次生代谢物质的作用。
方法将白背飞虱和红腹缢管蚜按不同比例混合饲养，待白背飞虱长至成虫后分组配对，分析白背飞虱总产卵量及日均产卵量；测定各处理的水稻草酸、黄酮及总酚含量，利用GC/MS仪器分析各处理水稻幼苗次生代谢物质成分的差异。
结果 “15头蚜虫 + 5头白背飞虱”处理的白背飞虱总产卵量仅为131.67粒，与“20头白背飞虱”处理(214.60粒)差异显著，日均产卵量也呈现相同的规律；“15头蚜虫 + 5头白背飞虱”处理的水稻黄酮和总酚含量分别为1.98和63.71 mg/L，均显著高于其他处理。GC/MS分析表明，“15头蚜虫 + 5头白背飞虱”处理的水稻中丙丁酚相对含量高达75.78%，而其余处理的水稻中不存在此种酚类物质。
结论红腹缢管蚜可能是通过刺激水稻提高黄酮及总酚含量，进而抑制白背飞虱的生殖能力，其中丙丁酚可能在白背飞虱和红腹缢管蚜种间互作中起着关键作用。

Abstract:
Objective To explore the interaction mechanism between species of Sogatella furcifera (white-backed planthopper) and Rhopalosiphum rufiabdominalis (aphid), and the role of secondary metabolites in rice during the interaction between the two insects.
Method S. furcifera and R. rufiabdominalis were mixed in different proportions, and the total egg production and average daily spawning of white-backed planthopper adults were analyzed after pairing. The contents of oxalic acid, flavones and total phenols of each treated rice seedlings were determined, and the differences in compositions of secondary metabolites were analyzed by GC/MS instrument.
Result The total egg production of white-backed planthoppers treated with “15 aphids + 5 white-backed planthoppers” was only 131.67 grains, which was significantly different from that of “20 white-backed planthoppers” (214.60 grains), and the average daily fecundity was the same. The contents of rice flavones and total phenols treated with “15 aphids + 5 white-backed planthoppers” were 1.98 and 63.71 mg/L, respectively, which were significantly higher than those of other treatments. GC/MS analysis showed that the relative content of probucol in rice treated with “15 aphids + 5 white-backed planthoppers” was as high as 75.78%, while this phenolic substance was not present in the rest of the treatments.
Conclusion R. rufiabdominalis may inhibit the reproductive ability of S. furcifera through stimulating rice to promote the content of flavones and total phenols. The probucol may play a key role in the interaction between S. furcifera and R. rufiabdominalis.

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