黄晓, 邓桂玲, 李发强. 基于细胞自噬的靶向降解技术及在植物研究中的应用前景[J]. 华南农业大学学报, 2022, 43(6): 97-106. DOI: 10.7671/j.issn.1001-411X.202208045
    引用本文: 黄晓, 邓桂玲, 李发强. 基于细胞自噬的靶向降解技术及在植物研究中的应用前景[J]. 华南农业大学学报, 2022, 43(6): 97-106. DOI: 10.7671/j.issn.1001-411X.202208045
    HUANG Xiao, DENG Guiling, LI Faqiang. Targeting autophagic degradation techniques and potential applications in plant science[J]. Journal of South China Agricultural University, 2022, 43(6): 97-106. DOI: 10.7671/j.issn.1001-411X.202208045
    Citation: HUANG Xiao, DENG Guiling, LI Faqiang. Targeting autophagic degradation techniques and potential applications in plant science[J]. Journal of South China Agricultural University, 2022, 43(6): 97-106. DOI: 10.7671/j.issn.1001-411X.202208045

    基于细胞自噬的靶向降解技术及在植物研究中的应用前景

    Targeting autophagic degradation techniques and potential applications in plant science

    • 摘要: 靶向降解技术是一类利用真核细胞内天然存在的降解机制对胞内有害物质进行特异降解、以维持和改善细胞稳态的重要技术。该技术主要通过泛素−蛋白酶体系统 (Ubiquitin-proteasome system,UPS) 和自噬−溶酶体途径 (Autophagy-lysosome pathway),特异性清除细胞内错误折叠或聚集的蛋白质、大分子复合物、受损或老化的细胞器及一些非蛋白类物质。其中基于细胞自噬的靶向降解技术具有专一性强、底物种类广泛等诸多特征,使其成为一备受期待的技术,有望应用于神经退行性疾病、代谢性疾病等多种疾病的治疗。目前这一技术的应用潜能还远未被完全开发,特别是在植物研究领域。本综述首先详细介绍了各类基于自噬−溶酶体途径的靶向降解技术的作用机制、特点以及优势;并且结合华南农业大学李发强教授课题组的研究工作,着重介绍了设计和改造植物选择性自噬衔接蛋白方面的研究和设想,以期达到将对植物生长发育不利的因子经由细胞自噬转运并区室化隔离于液泡的目的,进而开发能够抵御病毒侵染或抵抗有害物质的农作物新品种;最后展望了靶向自噬的降解技术在植物科学研究和农业生产中的潜在应用前景和所面临的挑战。

       

      Abstract: Targeted degradation techniques use the naturally existing degradation mechanism(s) in eukaryotic cells to specifically degrade harmful substances to maintain and improve cellular homeostasis. The techniques specifically remove misfolded or aggregated proteins, macromolecular complexes, damaged or aged organelles, and some non-protein substances via the ubiquitin-proteasome system (UPS) and the autophagy-lysosome pathway. Among these techniques, the targeting autophagic degradation techniques have many characteristics such as high target selectivity and wide substrate scope, making them promising techniques for the treatment of neurodegenerative diseases, metabolic diseases, and other diseases. At present, the application potential of these techniques is far from being fully developed, especially in the field of plant science. This review details the mechanisms, characteristics, and advantages of targeted degradation techniques based on the autophagy-lysosomal pathway. Moreover, combined with the recent research work of Professor Li Faqiang group in South China Agricultural University, this review focuses on the research of designing and modifying the autophagy adaptors to transport those factors unfavorable to plant growth and development via selective autophagy to the vacuole for compartmentation, so as to develop new crop varieties that can resist viral infection or toxic abiotic components. This review ends with a discussion of the potential applications and challenges of targeted autophagic degradation techniques in plant science and agricultural production.

       

    /

    返回文章
    返回