Abstract:
Objective To explore the genetic information of GmMADS4, a member of soybean (Glycine max) MADS transcription factor family, and analyze its structure and function.
Method The structure, coding protein information, conserved domain, phylogenetic tree analysis and interaction protein prediction of GmMADS4 were analyzed by bioinformatics analysis. Transient transformation of tobacco leaves was used to analyze subcellular localization, RT-qPCR was used to analyze its expression patterns in different tissues and response to nutrient deficiency, and hypocotyl complex plant transformation method was used to analyze the effects of overexpression of GmMADS4 on transgenic hair root growth.
Result The length of open reading frame of GmMADS4 was 732 bp and the relative molecular mass of coding protein was 28 000. The conserved domain of GmMADS4 contained MADS-box and K-box, which was a member of type II MADS family, and had a high similarity with Arabidopsis AtAP3. GmMADS4 was expressed in many parts of soybean, especially in flowers and seeds. The expression of GmMADS4 in leaves and roots significantly increased by nitrogen and phosphorus deficiency induction. GmMADS4 was mainly located in the nucleus, and overexpression of GmMADS4 significantly increased soluble phosphate content in transgenic hair roots.
Conclusion GmMADS4 is a member of soybean type II MADS family, and has nuclear localization function, which may play a role in the development of soybean seeds and flowers, as well as participate in soybean root adaptive responses to phosphorus deficiency and the regulation of phosphate homeostasis.
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