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    1株红树内生真菌高粱附球菌Epicoccum sorghinum的代谢产物及抗菌活性

    Metabolites and antifungal activities of an endophytic fungus Epicoccum sorghinum from mangrove

      摘要
    • 摘要:
      目的  研究红树内生真菌高粱附球菌Epicoccum sorghinum L28的代谢产物及其抗真菌活性。
      方法  柱层析分离纯化代谢产物，波谱技术鉴定结构；二倍稀释法测试代谢产物对小麦赤霉菌Fusarium graminearum和番茄枯萎菌F. oxysporum的最小抑菌浓度(Minimum inhibitory concentration，MIC)。
      结果  分离鉴定出7−羟基−2，5−二甲基色原酮(化合物1)、Livistone A(化合物2)、Barceloneic acid A(化合物3)、Barceloneic lactone(化合物4)、2′-O-methylbarceloneate(化合物5)、Spirostaphylotrichin R(化合物6)、 Spirostaphylotrichin U(化合物7)、β−谷甾醇(化合物8)、β−胡萝卜苷(化合物9)和β−腺苷(化合物10)。化合物1能强烈抑制小麦赤霉菌和番茄枯萎菌的生长，MIC均为100 μg/mL；化合物5对番茄枯萎菌的生长具有中等抑制作用，MIC为200 μg/mL。
      结论  化合物1和化合物5可作为相应抗菌农药先导化合物开展研究。

       

      Abstract:
      Objective  The metabolites of Epicoccum sorghinum L28 from mangrove and their antifungal activities were studied.
      Method  The metabolites were isolated and purified by column chromatography, and their structures were identified by spectral technique. The minimum inhibitory concentration (MIC) of the metabolites against Fusarium graminearum and F. oxysporum were tested by the double dilution method.
      Result  Ten compounds were isolated and identified as 7-hydroxy-2, 5-dimethyltrichroprimorone (compound 1), livistone A (compound 2), barceloneic acid A (compound 3), barceloneic lactone (compound 4), 2'-O-methylbarceloneate (compound 5), spirostaphylotrichin R (compound 6), spirostaphylotrichin U (compound 7), β-sitosterol (compound 8), β-carotenoside (compound 9) and β-adenosine (compound 10). 7-hydroxy-2, 5-dimethyltryptophanone displayed strong inhibitory effect against the growths of F. graminearum and F. oxysporum with MIC of 100 μg/mL. 2'-O-methylbarceloneate showed moderate inhibitory activity against F. oxysporum with MIC of 200 μg/mL.
      Conclusion  Compounds 1 and 5 could be further studied as the lead compounds of corresponding antifungal pesticides.

       

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