全氟辛酸对小鼠卵母细胞的毒性影响

郭聪慧; 李清华; 丁林舒; 黄建豪; 黄晓刚; 卫恒习; 张守全

doi:10.7671/j.issn.1001-411X.202101028

全氟辛酸对小鼠卵母细胞的毒性影响

Toxic effects of perfluorooctanoic acid on mouse oocytes

摘要

摘要:
目的研究全氟辛酸(Perfuorooctanoic aid，PFOA)对小鼠卵母细胞的毒性影响。

方法选用6周龄体质量相近的昆明雌性小鼠160只，分为4组，每组5个重复，每个重复8只小鼠。在适应环境7 d后开始对小鼠灌服不同剂量的PFOA，其中，对照组、低剂量组、中剂量组和高剂量组的每日剂量分别为0、 5、 10和20 mg/kg，连续灌服14 d，然后利用孕马血清促性腺激素(Pregnant mare serum gonadotropin，PMSG)和人绒毛促性腺激素(Human chorionic gonadotropin，hCG)对各组小鼠进行超数排卵，获取卵母细胞进行检测。

结果与对照组相比，PFOA中、高剂量组小鼠的卵母细胞成熟率分别下降了14.28%、28.17%；卵母细胞内活性氧含量分别升高了135%、177%；而且卵母细胞β-tubulin形态异常，染色体非整齐排列的比例分别升高了65.06%、75.60%。此外，低、中、高剂量组PFOA处理后小鼠的卵母细胞内磷酸化组蛋白H2AX(Phospho-histone H2A.X，P-H2A.X)比例分别比对照组升高了47%、133%和171%。

结论灌服PFOA可诱导小鼠卵母细胞内活性氧升高，DNA双链断裂和细胞骨架损伤，降低卵母细胞成熟率。

Abstract:
Objective The purpose of this study was to investigate the toxic effect of perfuorooctanoic aid(PFOA) on mouse oocytes.

Method One hundred and sixty 6-week-old Kunming female mice with similar body weight were divided randomly into four groups, with five replicates in each group and eight mice in each replicate. After 7 days of adaptation, the mice were fed with different doses of PFOA for 14 days. The daily doses for control, low, medium, and high dose groups were 0, 5, 10 and 20 mg/kg respectively. Then the mice were superovulated with injection of pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG), and the oocytes were obtained for detection.

Result Compared to the control group, the oocyte maturation rates of mice in the middle and high dose groups decreased by 14.28% and 28.17% respectively, while the contents of reactive oxygen species in oocytes increased by 135% and 177% respectively. The proportions of β-tubulin with abnormal morphology and irregular chromosomes in oocytes increased by 65.06% and 75.60% respectively. In addition, the proportions of (Phospho-histone H2A.X, P-H2A.X) in oocytes of low, medium and high dose groups increased by 47%, 133% and 1.71% respectively compared with the control.

Conclusion Mice fed with PFOA may decrease their oocyte maturation rate by inducing the increase of reactive oxygen species, DNA double strand break and cytoskeleton damage in oocytes.

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