恩诺沙星纳米乳的制备及喷雾给药的药效学评价

许颖; 邱阳阳; 沈悦; 冯慧心; 冯云云; 黄显会

doi:10.7671/j.issn.1001-411X.202001030

恩诺沙星纳米乳的制备及喷雾给药的药效学评价

Preparation of enrofloxacin nanoemulsion and evaluation of pharmacodynamic of spray administration

摘要

摘要:
目的以恩诺沙星为主药，研制一种可喷雾给药的纳米乳新制剂，并通过鸡全身感染疾病模型，对恩诺沙星纳米乳喷雾制剂的药效进行初步评价。

方法采用相转变法，通过单因素试验和正交试验对辅料进行筛选及处方工艺优化，采用高效液相色谱法(HPLC)对纳米乳中恩诺沙星的含量进行测定，采用纳米粒度及Zeta电位分析仪测定纳米乳粒径大小和Zeta电位，并开展人工诱发鸡白痢沙门氏菌病和禽大肠埃希菌病感染模型的药效学试验，评估恩诺沙星纳米乳喷雾给药对常见细菌性疾病的防治效果。

结果本试验所制备的纳米乳为水包油型，呈淡黄色，澄清透明，乳滴分布均匀，平均粒径为（10.79±1.5） nm，多分散系数(PDI)为0.446，Zeta电位为−21.63 mV。药效学评估显示：高、中、低剂量预防给药组的存活率分别为90.0%、83.3%、73.3%，均显著高于感染对照组(26.7%)(P<0.01)。

结论本研究制备的复方恩诺沙星纳米乳性质稳定，超声雾化喷雾给药对沙门氏菌和大肠埃希菌引起的鸡全身感染有良好的防治效果。

Abstract:
Objective To develop a new formulation of enrofloxacin nanoemulsion for spray administration, and evaluate its disease control effect using chicken model of systemic infection.

Method The phase transition method was used, and single factor and orthogonal tests were performed to screen formulations and optimize the prescription. High-performance liquid chromatography (HPLC) was used to determine enrofloxacin content in nanoemulsion. Nanoparticle and zeta potential analyzer was used to measure the particle size and zeta potential of nanoemulsion. Pharmacodynamic experiments were performed using chicken models infected by Salmonella pullorum and Escherichia coli, and the control effects of spray adminstration of compound enrofloxacin nanoemulsion on bacterial diseases were evaluated.

Result The nanoemulsion prepared in this experiment was an oil-in-water type, light yellow, clear and transparent, and had uniform droplet distribution. The average particle size was (10.79 ± 1.5) nm, the polydispersity index (PDI) was 0.446 and the zeta potential was −21.63 mV. The pharmacodynamic evaluation showed that the survival rates of preventive administration groups with high, medium and low doses were 90.0%, 83.3% and 73.3% respectively, which were significantly higher than that of the infection control group (26.7%) ( P<0.01).

Conclusion The compound enrofloxacin nanoemulsion prepared in this study has stable properties. The ultrasonic spray administration has good control effects on chicken diseases due to systemic infection of Salmonella pullorum and Escherichia coli.

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