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    组氨酸激酶基因envZ调控禽致病性大肠埃希菌生物被膜的机制研究

    Mechanism of histidine kinase gene envZ regulating biofilm of avian pathogenic Escherichia coli

      摘要
    • 摘要:
      目的  研究双组分系统ompR/envZ中的组氨酸激酶基因envZ对禽致病性大肠埃希菌(Avian pathogenic Escherichia coli，APEC)生物被膜形成能力的影响，了解该双组分系统在APEC中对生物被膜的调控机制，为探索生物被膜影响禽致病性大埃希菌耐药性的途径提供参考。
      方法  采用Red同源重组的方法构建envZ基因缺失株，比较野生株和envZ基因缺失株生长特性、生物被膜形成能力的差异。利用转录组学方法分析envZ在转录调控网络中对其生物被膜形成相关基因的调控机制。
      结果  成功构建envZ基因缺失株AE17ΔenvZ；envZ基因缺失对APEC的生长速度无明显影响，但使APEC生物被膜的成膜能力减弱。与野生株AE17相比，envZ基因缺失株有711个基因发生差异表达，与生物被膜形成相关的基因显著下调。
      结论  envZ基因除了响应环境渗透压，还参与调控APEC生物被膜的形成。

       

      Abstract:
      Objective  To study the effect of histidine kinase gene envZ in two-component system ompR/envZ on biofilm formation ability of avian pathogenic Escherichia coli (APEC), understand the regulation mechanism of the two-component system on biofilm of APEC, and provide a reference for investigating the ways of biofilms affecting APEC antibiotic resistance.
      Method  The red homologous recombination method was used to construct the envZ-gene-deleted strain, and the differences in growth characteristics and biofilm formation abilities between wild strain and the envZ-gene-deleted strain were compared. The mechanism of envZ regulating biofilm formation related genes in transcriptional regulatory network was analyzed by transcriptomics.
      Result  The envZ-gene-deleted strain AE17ΔenvZ was successfully constructed. The envZ gene deletion had no significant effect on growth rate of APEC, but weakened the APEC biofilm formation ability. Compared with wild strain AE17, AE17ΔenvZ had 711 differentially expressed genes, and the genes related to biofilm formation were significant down regulation.
      Conclusion  In addition to responding to environmental osmotic pressure, envZ gene is also involved in regulating the formation of APEC biofilm.

       

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