入侵性食叶害虫椰子织蛾的单倍型多样性分析

李优佳; 杨帆; 吕宝乾; 何荣晓; 蔡波

doi:10.7671/j.issn.1001-411X.201910023

入侵性食叶害虫椰子织蛾的单倍型多样性分析

Analysis of haplotype diversity of an invasive leaf-eating pest Opisina arenosella

摘要

摘要:
目的分析危害棕榈科Palmae植物的一种重要入侵食叶害虫椰子织蛾Opisina arenosella 单倍型在原产地和入侵地的分布特点，揭示椰子织蛾入侵我国的虫源信息。

方法利用线粒体COI基因分析16个地理种群共计172个样本，比较椰子织蛾印度种群和入侵地(中国、马来西亚和泰国)种群的遗传关系。

结果片段长度为625 bp的172条序列共鉴定出12个单倍型，包含15个变异位点，构成2个明显的单倍型分支，其中一个分支由11个单倍型(IN1~IN11)组成，均来自印度种群，单倍型IN1是6个印度种群的共享单倍型，IN2~IN11为独享单倍型；另一个分支为单倍型HAP，由来自中国、马来西亚和泰国的种群共享；HAP与11个来自印度的单倍型IN1~IN11均存在4个变异位点。

结论入侵地区的椰子织蛾种群来自同一基因型或者具有相同的入侵源；椰子织蛾种群入侵后受环境选择压力，在新栖息地产生新的突变或杂交。

Abstract:
Objective Opisina arenosella is an important invasive leaf-eating pest that attack Palmae plants. The goal was to analyze the distribution characteristics of haplotypes of O. arenosella in native and invaded zones, and reveal the insect source information of O. arenosella invading China.

Method A total of 172 samples from 16 geographical populations were analyzed by mitochondrial COI gene, and the genetic relationship of O. arenosella from India and invading areas (China, Malaysia and Thailand) was compared.

Result Twelve haplotypes were identified in 172 sequences with fragment length of 625 bp. Fifteen variation sites were detected in the haplotype alignment. Two obvious haplotype branches were formed, one of which was composed of 11 haplotypes IN1-IN11 and they were all from Indian populations. Haplotype IN1 was shared by six O. arenosella populations from India. IN2-IN11 were exclusive haplotypes and not shared with other populations. The other branch was haplotype HAP and shared by populations from China, Malaysia and Thailand. There were four variation sites between HAP and 11 haplotypes IN1-IN11 from India.

Conclusion O. arenosella populations in these invading areas are from the same genotype type or have the same invasion source. O. arenosella invading populations produce new mutations or hybrids in new habitats under environmental selection pressure.

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