噻虫嗪对褐飞虱的毒力及解毒代谢酶活性的影响

张钰明; 向兴; 王学贵

doi:10.7671/j.issn.1001-411X.201908024

噻虫嗪对褐飞虱的毒力及解毒代谢酶活性的影响

四川农业大学农学院/国家级作物学教学实验示范中心，四川成都 611130

基金项目: 国家重点研发计划(2018YFD0200300)

详细信息

作者简介:
张钰明(1997—)，女，硕士研究生，E-mail: m18408215896@163.com

通讯作者:
王学贵(1976—)，男，副教授，博士，E-mail: wangxuegui@sicau.edu.cn

中图分类号: Q965.9
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出版历程
- 收稿日期: 2019-08-18
- 网络出版日期: 2023-05-17
- 刊出日期: 2020-05-09

Effects of thiamethoxam on toxicity and detoxification metabolic enzyme activity of Nilaparvata lugens

College of Agriculture, Sichuan Agricultural University/National Demonstration Center for Experimental Grop Science Education, Chengdu 611130, China

摘要

摘要:
目的
明确噻虫嗪对褐飞虱Nilaparvata lugens的抗药性及解毒代谢酶活性的影响。

方法
采用稻苗浸渍法测定褐飞虱温室种群对吡虫啉、噻虫嗪、噻嗪酮和毒死蜱的抗药性，以及对新型防控药剂氟啶虫胺腈和三氯苯嘧啶的敏感性；研究增效剂胡椒基丁醚 (PBO)、马来酸二乙酯 (DEM)和磷酸三苯酯 (TPP)对噻虫嗪的增效作用；测定了羧酸酯酶、谷胱甘肽S−转移酶和细胞色素 P450s 酶活性。

结果
褐飞虱种群对噻虫嗪表现出高水平抗性，抗性倍数达到277.92倍，对氟啶虫胺腈和三氟苯嘧啶仍处于敏感水平，对三氟苯嘧啶无交互抗性。PBO对噻虫嗪的增效作用最强，增效倍数为1.99倍。温室抗性种群的细胞色素P450s活性达到4.70×10⁻³ IU/mg，为室内敏感品系的2.13倍。

结论
细胞色素P450s活性的增强可能是褐飞虱对噻虫嗪产生代谢抗性的主要原因。氟啶虫胺腈和三氟苯嘧啶轮换使用可有效防控褐飞虱。
- 褐飞虱 /
- 稻苗浸渍法 /
- 噻虫嗪 /
- 胡椒基丁醚 /
- P450s
Abstract:
Objective
To clarify the effects of thiamethoxam on resistance and detoxification metabolic enzyme activity of Nilaparvata lugens.

Method
The resistance levels of N. lugens population in greenhouse against four insecticides (imidacloprid, thiamethoxam, buprofezin, chlopyrifos) and the sensitivities to two new types of insecticides (sulfoxaflor, triflumezopyrim) were measured using rice seedling immersion method. The synergistic effect of three synergists [piperonyl butoxide (PBO), diethyl maleate (DEM), triphenyl phosphate (TPP)] on thiamethoxazine, and three detoxification enzymes (carboxylesterase, qlutathioneS-transferase, cytochrome P450s) activities were analyzed.

Result
N. lugens population showed high resistance to thiamethoxam with the resistance ratio of 277.92 folds, sensitive to sulfoxaflor and triflumezopyrim, and no cross resistance to triflumezopyrim. PBO had the strongest synergistic effect on thiamethoxam with the synergistic ratio of 1.99. Cytochrome P450s activity of greenhouse population reached 4.70×10⁻³ IU/ mg, which was 2.13 folds to that of susceptible strain.

Conclusion
The increased activity of cytochrome P450s could be the main factor resulting in the metabolic resistance of N. lugens to thiamethoxam. The alternate use of sulfoxaflor and triflumezopyrim can effectively control the occurrence of N. lugens.
- Nilaparvata lugens /
- rice seedling immersion method /
- thiamethoxam /
- piperonyl butoxide /
- P450s

HTML全文

图 1 不同增效剂处理下褐飞虱的解毒代谢酶活力

图中S、G分别为敏感和温室种群；TPP、DEM、PBO分别为磷酸三苯酯、马来酸二乙酯和胡椒基丁醚

Figure 1. Detoxification enzyme activities of Nilaparvata lugens under different synergists

S: Sensitive population, G: Greenhouse population; TPP: Triphenyl phosphate; DEM: Diethyl maleate; PBO: Piperonyl butoxide

下载: 全尺寸图片幻灯片

表 1 6种杀虫剂对褐飞虱的毒力

Table 1 The toxicities of six insecticides on Nilaparvata lugens

药剂名称 Insecticide	处理种群 Population	斜率±标准误 Slope±SE	LC₅₀/ (μg·mL⁻¹)	95%置信区间¹⁾/(μg·mL⁻¹) 95% confidence interval	χ² (df)	抗性倍数 Resistance ratio
毒死蜱 Chlorpyrifos	敏感基线^[10] Sensitive baseline	4.259±3.144	1.721	1.400~12.810		1.00
毒死蜱 Chlorpyrifos	温室种群 Greenhouse population	3.148±0.614	16.613	11.841~20.580	13.74(16)	9.65
吡虫啉 Imidacloprid	敏感基线^[10] Sensitive baseline	6.677±1.512	0.078	0.050~0.100		1.00
吡虫啉 Imidacloprid	温室种群 Greenhouse population	2.666±0.332	148.399	118.147~180.048	11.35(13)	1 902.55
噻虫嗪 Thiamethoxam	敏感基线^[10] Sensitive baseline	7.134±2.184	0.105	0.090~0.120		1.00
噻虫嗪 Thiamethoxam	温室种群 Greenhouse population	2.895±0.353	29.182	23.613~35.186	10.30(13)	277.92
噻嗪酮 Buprofezin	敏感基线^[10] Sensitive baseline	10.019±4.248	0.066	0.060~0.070		1.00
噻嗪酮 Buprofezin	温室种群 Greenhouse population	2.006±0.291	56.500	43.570~73.084	10.50(13)	856.06
三氟苯嘧啶 Triflumezopyrim	敏感品系 Susceptible strain	1.668±0.308	0.111	0.060~0.155	7.23(13)	1.00
三氟苯嘧啶 Triflumezopyrim	温室种群 Greenhouse population	1.778±0.300	0.205	0.118~0.287	11.02(13)	1.85
氟啶虫胺腈 Sulfoxaflor	敏感品系 Susceptible strain	3.115±0.391	1.013	0.834~1.175	10.34(16)	1.00
氟啶虫胺腈 Sulfoxaflor	温室种群 Greenhouse population	4.154±0.741	3.293	2.564~3.844	10.28(13)	3.25
1)LC₅₀值95%置信区间不重叠视为差异显著　1) LC₅₀ values are considered significantly different when the 95% confidence intervals do not overlap

下载: 导出CSV

表 2 3种增效剂对噻虫嗪的增效作用

Table 2 Synergistic effects of three synergists on thiamethoxam

处理种群 Population	处理 Treatment	斜率±标准误 Slope±SE	LC₅₀ / (μg·mL⁻¹)	95%置信区间¹⁾/(μg·mL⁻¹) 95% confidence interval	χ² (df)	增效倍数 Synergism ratio
敏感品系 Susceptible strain	噻虫嗪 Thiamethoxam	2.930±0.280	2.589	2.201~3.030	6.36(16)	1.00
	噻虫嗪+TPP Thiamethoxam+TPP	2.269±0.228	2.410	1.994~2.898	14.39(16)	1.07
	噻虫嗪+PBO Thiamethoxam+PBO	2.900±0.277	2.262	1.921~2.653	8.71(16)	1.14
	噻虫嗪+DEM Thiamethoxam+DEM	2.871±0.275	2.489	2.114~2.920	7.34(16)	1.04
温室种群 Greenhouse population	噻虫嗪 Thiamethoxam	2.745±0.391	27.073	20.467~33.345	13.3(13)	1.00
	噻虫嗪+TPP Thiamethoxam+TPP	2.499±0.249	19.357	15.400~23.925	21.6(16)	1.40
	噻虫嗪+PBO Thiamethoxam+PBO	2.271±0.259	13.575	10.601~16.666	13.4(16)	1.99
	噻虫嗪+DEM Thiamethoxam+DEM	2.590±0.297	21.128	17.025~25.394	13.19(16)	1.28
1)LC₅₀值95%置信区间不重叠视为差异显著　1)LC₅₀ values are considered significantly different when the 95% confidence intervals do not overlap

下载: 导出CSV

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