展颖野生稻单片段代换系芽期耐Cd材料筛选及QTL鉴定

王璋强; 李蕊芯; 陆怡; 杨帆; 刘田田; 何平; 张鸿兴; 熊毅; 傅雪琳

doi:10.7671/j.issn.1001-411X.201905023

展颖野生稻单片段代换系芽期耐Cd材料筛选及QTL鉴定

Cd-tolerant germplasm screen and QTL identification of single-segment substitution lines of Oryza glumaepatula at the germination period

摘要

摘要:
目的评价CdCl₂溶液对展颖野生稻Oryza glumaepatula单片段代换系(SSSLs)种子发芽及根芽生长的影响，筛选耐Cd SSSLs材料，初步鉴定与芽期耐Cd性状相关的QTLs。

方法对6个栽培稻品种在0、30、50、100、500 μmol·L⁻¹ CdCl₂溶液条件下进行发芽试验，确定水稻种子芽期耐Cd性研究的处理浓度，筛选耐Cd SSSLs的性状指标。对SSSLs在CdCl₂溶液处理下发芽第7天的根长与芽长分析，筛选P=0.01水平耐Cd性较强的SSSLs，鉴定相应性状QTLs。

结果在0、30、50和100 μmol·L⁻¹ CdCl₂浓度处理下，栽培稻品种的种子发芽率为91%~95%，差异不显著，但种子根和幼芽伸长生长受到抑制，品种间差异显著。在50和100 μmol·L⁻¹ CdCl₂溶液条件下，以发芽第7天的相对根长和相对芽长为指标，在P=0.01水平从8个SSSLs鉴定出5个相对根长QTLs，即qRRL1-1、qRRL2-1、qRRL3-1、qRRL3-2及qRRL6-1，其加性效应为0.19~0.60，表型贡献率为31.18%~100.59%；鉴定出5份SSSLs携带3个相对芽长QTLs，即qRSL1-1、qRSL1-2及qRSL2-1，其加性效应为0.08~0.18，表型贡献率为7.43%~18.95%。

结论展颖野生稻SSSLs携带芽期耐Cd QTLs，可以作为水稻耐Cd基因发掘的重要材料。

Abstract:
Objective To evaluate effects of Cd stress on seed germination and growth of seminal root and shoot in chromosome single-segment substitution lines (SSSLs) of Oryza glumaepatula after seed germination, screen for Cd-tolerant SSSLs, and identify QTLs related to Cd-tolerant characters in bud.

Method The seed germination test was carried out using six varieties at CdCl₂ concentrations of 0, 30, 50, 100, 500 μmol·L⁻¹, and the proper treatment of CdCl₂ concentration and the related character indexes to evaluate CdCl₂ tolerance of SSSLs were determined. The lengths of seminal roots and shoots of experimental SSSLs were analyzed to screen for SSSLs with strong tolerance to CdCl₂ at P=0.01 level and identify QTLs related to Cd-tolerant characters.

Result At the concentrations of 0, 30, 50, and 100 μmol·L⁻¹ CdCl₂, the seed germination rates of six varieties were distributed between 91%～95%, which were insignificant among different CdCl₂ concentrations. However, the growth of seminal roots and shoots were significantly inhibited by CdCl₂, and the significant differences were observed for the tolerance to CdCl₂ among different varieties. Using relative root length and relative shoot length on the 7th day after germination as tolerance indexes, at the concentrations of 50 and 100 μmol·L⁻¹, five relative root length QTLs including qRRL1-1, qRRL2-1, qRRL3-1, qRRL3-2 and qRRL6-1 were identified in eight SSSLs at P=0.01 level, the phenotypic contribution rates of additive effects ranged from 31.18% to 100.59%. Three relative shoot length QTLs including qRSL1-1, qRSL1-2, and qRSL2-1 were identified in five SSSLs, the phenotypic contribution rates of additive effects were 7.43%～18.95%.

Conclusion SSSLs of O. glumaepatula carry Cd-tolerant QTLs at the germination period, which will be useful for exploring beneficial Cd-tolerant genes in rice.

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