Effects of calcium chloride supplementation in drinking water on fat deposition and intestinal flora in mice fed with high-fat diet
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摘要:目的
研究饮水添加氯化钙对高脂日粮饲喂小鼠脂肪沉积和肠道菌群的影响，为提高动物胴体品质和人体健康提供理论依据。
方法选用27只4周龄C57BL雄性小鼠，分为高脂日粮组和高脂日粮+饮水添加氯化钙组，试验期13周。每周测定小鼠体质量，试验末测定小鼠体脂含量，试验结束后采集小鼠皮下脂肪、附睾脂肪并称质量。于试验后期10~12周采集小鼠粪便进行16S rRNA高通量测序，并分析对菌群的影响。
结果饮水添加氯化钙显著降低了小鼠体质量、体脂含量、皮下脂肪指数和附睾脂肪指数，与高脂组相比分别降低了12.85%、32.69%、26.65%和18.60%。饮水添加氯化钙提高了小鼠粪便样中的菌群多样性和菌群丰度。在门水平下，饮水添加氯化钙对拟杆菌门Bacteroidetes、厚壁菌门Firmicutes、变形菌门Proteobacteria、脱铁杆菌门Deferribacteres、放线菌门Actinobacteria、软壁菌门Tenericutes的丰度无明显影响。在纲水平下，与高脂组相比，饮水添加氯化钙显著降低了丹毒丝菌纲Erysipelotrichia和放线菌纲Actinobacteria的相对丰度，并且显著提高了梭菌纲Clostridia的相对丰度。
结论饮水添加氯化钙能够降低高脂日粮饲喂小鼠的体质量和体脂含量，这可能与饮水添加氯化钙提高了小鼠粪便菌群多样性和特定菌群丰度有关。
Abstract:ObjectiveTo investigate the effects of calcium chloride supplementation in drinking water on fat deposition and intestinal flora in mice fed high-fat diet, and provide a theoretical basis for improving carcass characteristics of animals and human health.
MethodTwenty-seven 4-week-old C57BL male mice were divided into two groups including a high-fat diet (HFD) group and a group with HFD+calcium chloride supplementation in drinking water. The experiment was conducted for 13 weeks. The body weight of mouse was measured weekly. The body fat content of mouse was determined at the end of the experiment. After mice were sacrificed, the subcutaneous fat and epididymal fat of mice were collected and weighed. Mouse feces were collected during 10−12 weeks for high-throughput sequencing of 16S rRNA and the effects on intestinal flora were analyzed.
ResultCalcium chloride supplementation in drinking water significantly reduced the body weight, body fat content, subcutaneous fat index and epididymal fat index of mice, and compared with HFD group these indexes were reduced by 12.85%, 32.69%, 26.65% and 18.60%, respectively. Meanwhile, calcium chloride supplementation in drinking water increased the community diversity and bacterial abundance in mouse fecal samples. At the phylum level, calcium chloride supplementation had no effect on the abundance of Bacteroidetes, Firmicutes, Proteobacteria, Deferribacteres, Actinobacteria and Tenericumes. At the class level, compared with the HFD group, calcium chloride supplementation in drinking water significantly reduced the relative abundance of Erysipelotrichia and Actinobacteria, whereas significantly increased the relative abundance of Clostridia.
ConclusionCalcium chloride supplementation in drinking water reduces the body weight and body fat content of mouse fed HFD, which might be associated with the increase in bacterial diversity and abundance of some specific bacteria in mouse feces.
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Keywords:
- calcium chloride /
- high-fat diet /
- mouse /
- fat deposition /
- intestinal flora
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图 1 饮水添加氯化钙对高脂日粮饲喂小鼠体质量(a)、体脂含量(b)、皮下脂肪指数(c)和附睾脂肪指数(d)的影响
“*” 和 “**” 分别表示与HFD组差异达到0.05和0.01的显著水平(t检验)
Figure 1. Effects of calcium chloride supplementation in drinking water on body weight (a), body fat content (b), subcutaneous fat index (c) and epididymal fat index (d) of mice fed high-fat diet
“*” and “**” indicate the significant difference between HFD and HFD+Ca2+ at 0.05 and 0.01 levels, respectively (t test)
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图 2 饮水添加氯化钙对高脂日粮饲喂小鼠粪便菌群的香农指数(a)及Chao指数(b)的影响
Figure 2. Effects of calcium chloride supplementation in drinking water on Shannon index (a) and Chao index (b) of mice fed high-fat diet
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