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    2017年福建地区PRRSV分子检测及ORF5基因变异分析

    Molecular detection of PRRSV and variation analysis of ORF5 gene in Fujian province in 2017

      摘要
    • 摘要:
      目的  研究2017年福建地区中小规模猪场猪繁殖与呼吸综合征病毒(PRRSV)毒株的分子流行情况。
      方法  从福建地区中小规模猪场收集83份疑似蓝耳阳性病料，对编码GP5蛋白的ORF5基因进行了RT-PCR检测和遗传进化分析。
      结果  采集的83份疑似蓝耳病料中阳性率为73%，测得的序列之间ORF5的核苷酸相似性达80.3%~100.0%，其中所测的各个分支毒株与其所属分支的代表毒株NADC30、GM2和JXA1的核苷酸相似性分别为92.6%~93.9%、89.8%~93.0%和93.4%~99.3%，检测到新分支中新出现的毒株与JXA1的核苷酸相似性为91.0~93.0%。GP5氨基酸比对结果显示不同亚群GP5信号肽区、诱导表位、主要中和表位及高变区等功能区发生了显著变异。
      结论  福建地区出现了PRRSV新分支，使福建PRRSV变异种类更加多样化，建议加强PRRSV流行及变异的监测。

       

      Abstract:
      Objective  To study the molecular prevalence of the porcine peproductive and respiratory syndrome virus (PRRSV) strain in small and medium-sized pig farms of Fujian province in 2017.
      Method  We collected 83 suspected blue ear positive disease materials from small and medium-sized pig farms in Fujian province. RT-PCR detection and genetic evolution analysis of ORF5 gene which encoded GP5 protein was performed.
      Result  The positive rate of 83 suspected blue ear disease samples was 73%, and the nucleotide similarity among the measured ORF5sequences was 80.3%−100.0%. The nucleotide similarities between tested strains of different branches and the representative strains of those branches, NADC30, GM2 and JXA1, were 92.6%−93.9%, 89.8%−93.0% and 93.4%−99.3%, respectively. The new identified strain in the new branch had nucleotide similarity of 91.0%−93.0% to JXA1. The GP5 amino acid alignment showed significant variation in functional regions such as GP5 signal peptide region, induction epitope, principal neutralizing epitope and hypervariable region in different subgroups.
      Conclusion  A new branch of PRRSV has emerged in Fujian area, making the PRRSV variants in Fujian more diverse. It is necessary to strengthen the monitoring of PRRSV epidemics and mutations.

       

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