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    张子琪, 吴旻, 兰海楠, 等. 虾青素缓解脂多糖诱导的小鼠急性肝损伤[J]. 华南农业大学学报, 2019, 40(1): 40-45. DOI: 10.7671/j.issn.1001-411X.201805004
    引用本文: 张子琪, 吴旻, 兰海楠, 等. 虾青素缓解脂多糖诱导的小鼠急性肝损伤[J]. 华南农业大学学报, 2019, 40(1): 40-45. DOI: 10.7671/j.issn.1001-411X.201805004
    shu
    ZHANG Ziqi, WU Min, LAN Hainan, et al. Astaxanthin alleviates lipopolysaccharide-induced acute liver injury of mice[J]. Journal of South China Agricultural University, 2019, 40(1): 40-45. DOI: 10.7671/j.issn.1001-411X.201805004
    Citation: ZHANG Ziqi, WU Min, LAN Hainan, et al. Astaxanthin alleviates lipopolysaccharide-induced acute liver injury of mice[J]. Journal of South China Agricultural University, 2019, 40(1): 40-45. DOI: 10.7671/j.issn.1001-411X.201805004
    shu

    虾青素缓解脂多糖诱导的小鼠急性肝损伤

    Astaxanthin alleviates lipopolysaccharide-induced acute liver injury of mice

      摘要
    • 摘要:
      目的  研究虾青素(AST)对脂多糖(LPS)诱导的小鼠急性肝损伤的影响。
      方法  健康雄性ICR小鼠40只随机分为4组, 包括对照组、AST组、LPS组和虾青素预保护组(AST+LPS组)。记录小鼠体质量并计算肝脏系数；通过ELISA法检测血清中髓过氧化物酶(MPO)含量；生物化学法测定肝组织中丙二醛(MDA)的含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)的活性；荧光定量PCR法检测抗氧化酶SOD、GSH-Px、CAT、谷氨酸半胱氨酸连接酶催化亚基(GCLC)的mRNA相对表达量；通过HE染色观察各组肝脏细胞形态和肝损伤程度。
      结果  各组小鼠初始体质量均为18 g，末次称量与初始体质量相比增加9~11 g，但各组间体质量增加无显著性差异(P>0.05)。与LPS组相比，AST+LPS组小鼠肝脏系数(0.054)、血清MPO质量浓度(10.20 ng·mL–1)和肝组织中MDA质量摩尔浓度(2.83 μmol·g–1)显著降低(P<0.05)，抗氧化酶SOD(512.14 U·mg–1)、GSH-Px(848.91 U·mg–1)和CAT(61.53 U·mg–1)活性显著提高(P<0.05)，抗氧化酶mRNA的相对表达量均显著升高(P<0.05)，同时肝脏损伤程度低，肝细胞形态完整，排列均匀。
      结论  虾青素可保护小鼠肝细胞形态，提高肝脏抗氧化水平，调节肝组织中抗氧化酶mRNA的表达，从而缓解LPS引起的肝脏氧化应激，减轻急性肝损伤。

       

      Abstract:
      Objective  To investigate the effect of astaxanthin (AST) treatment on acute liver injury induced by lipopolysaccharide (LPS) in mouse.
      Method  Forty healthy male ICR mice were randomly allocated into four groups including control group(CK), AST group, LPS group and AST preprotection group (AST+LPS group). Body weight and liver index of mice were recorded. Myeloperoxidase (MPO) level in serum was measured by ELISA. Malondialdehyde (MDA) content and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-px) and catalase(CAT) were measured by biochemical methods. The relative mRNA expression levels of antioxidant enzymes including SOD, GSH-Px, CAT and glutamate cysteine ligase catalytic subunit (GCLC) were measured by fluorescence quantitative PCR. HE staining was used to observe the histopathological changes.
      Result  The initial weight of mice in each group was 18 g. The final weight was 9−11 g higher than the initial weight, and there was no significant difference among groups(P>0.05). Compared with LPS group, the liver index (0.054), serum MPO level (10.20 ng·mL–1), and MDA content (2.83 μmol·g–1) in liver tissue were significantly reduced in AST+LPS group (P<0.05). Astaxanthin increased the activities of SOD(512.14 U·mg–1), GSH-Px(848.91 U·mg–1) and CAT (61.53 U·mg–1) as well as the relative mRNA expression levels of tested antioxidases. In addition, the damage degree of liver in AST+LPS group was low, and hepatocyte structure was perfectly aligned.
      Conclusion  Astaxanthin treatment can protect the morphology of hepatocyte, increase antioxidant level and the mRNA expression of antioxidase in liver, and thereby relive liver oxidative stress and alleviate LPS-induced acute liver injury in mice.

       

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