乙烯和1-MCP对白姜花萜类香气及相关基因表达的影响

刘晓洲; 范燕萍; 余让才; 岳跃冲; 李昕悦; 玉云祎

doi:10.7671/j.issn.1001-411X.2018.04.011

乙烯和1-MCP对白姜花萜类香气及相关基因表达的影响

刘晓洲^{1, 2,},
范燕萍^{1, 2, ,},
余让才²,
岳跃冲^{1, 2},
李昕悦^{1, 2},
玉云祎^{1, 2}

1.
华南农业大学林学与风景园林学院，广东广州 510642
2.
华南农业大学花卉研究中心，广东广州 510642

基金项目: 国家自然科学基金(31770738，313370694)

详细信息

作者简介:
刘晓洲(1992—)，男，硕士研究生，E-mail:624546499@qq.com

通讯作者:
范燕萍(1962—)，女，教授，博士，E-mail: fanyanping@scau.edu.cn

中图分类号: S682.1
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出版历程
- 收稿日期: 2018-01-18
- 网络出版日期: 2023-05-17
- 刊出日期: 2018-07-09

Effect of ethylene and 1-MCP on the terpenoid fragrance of Hedychium coronarium and the expressions of related genes

LIU Xiaozhou^{1, 2,},
FAN Yanping^{1, 2, ,},
YU Rangcai²,
YUE Yuechong^{1, 2},
LI Xinyue^{1, 2},
YU Yunyi^{1, 2}

1.
College of Forestry and Landscape Archetecture, South China Agricutural University, Guangzhou 510642, China
2.
Guangzhou Flower Research Center, South China Agricutural University, Guangzhou 510642, China

摘要

摘要:
目的
研究乙烯和1-MCP对白姜花萜类香气物质合成释放的影响，探究相关基因的表达变化。

方法
以白姜花Hedychium coronarium为材料，分别用10 μL·L^–1的乙烯和4 μL·L^–1的乙烯抑制剂1-MCP处理8 h，随后采用顶空固相微萃取和气相色谱–质谱(GC-MS)技术进行挥发性香气物质的测定，所得到的离子图用NIST 08数据库进行定性及定量分析。通过荧光定量PCR分析相关基因的表达情况。

结果
乙烯处理后白姜花香气成分罗勒烯、沉香醇、别罗勒烯、石竹烯和法尼烯的挥发量显著上升，相关香气萜类合成酶基因HcTPS1、HcTPS3和HcTPS10的表达量分别上升了116%、182%和63%；而用1-MCP处理后，罗勒烯、别罗勒烯和法尼烯的挥发量显著下降，HcTPS1、HcTPS3和HcTPS10基因的表达量分别降低了45%、30%和30%。乙烯处理后，核转录因子HcEIL1-1和HcEIL1-2的表达量分别上升了51%和114%；1-MCP处理后，HcEIL1-1和HcEIL1-2表达量分别下降了30%和60%。

结论
外源乙烯可以提升白姜花萜类花香物质的释放量，白姜花乙烯信号通路中的核转录因子HcEIL1-1和HcEIL1-2与萜类花香合成酶基因的表达模式一致。
- 白姜花 /
- 香气 /
- 乙烯 /
- 1-MCP /
- 基因表达 /
- 萜类化合物
Abstract:
Objective
To investigate the effect of ethylene and 1-methylcyclopropene (1-MCP) treatment on the terpenoid fragrance of Hedychium coronarium and analyze the expressions of related genes.

Method
H. coronarium was treated separately with 10 μL·L^–1 ethylene and 4 μL·L^–1 ethylene inhibitor 1-MCP for 8 h. Volatile scent compounds were measured by headspace solid-phase microextraction and gas chromatography-mass spectrometry (GC-MS), and the obtained ion chromatograms were analyzed using NIST 08 database. The expressions of related genes were analyzed by fluorescence quantitative PCR.

Result
After ethylene treatment, the emissions of volatile scent compounds ocimene, linalool, allo-ocimene, β-caryophyllene and α-farnesene increased significantly, and the expressions of related terpene synthase genes HcTPS1, HcTPS3 and HcTPS10 increased by 116%, 182% and 63% respectively. After 1-MCP treatment, the emissions of ocimene, allo-ocimene and α-farnesene decreased significantly, and the expressions of HcTPS1, HcTPS3 and HcTPS10 decreased by 45%, 30% and 30% respectively. After ethylene treatment, the expressions of nuclear transcription factors HcEIL1-1 and HcEIL1-2 increased by 1.51 and 2.14 times respectively. After 1-MCP treatment, the expressions of HcEIL1-1 and HcEIL1-2 decreased by 30% and 60% respectively.

Conclusion
Exogenous ethylene can increase the emission of terpenoids in H. coronarium. The expression patterns of HcEIL1-1 and HcEIL1-2, the nuclear transcription factors in ethylene signaling pathway, were consistent with the expression pattern of terpenoid synthase genes.
- Hedychium coronarium /
- fragrance /
- ethylene /
- 1-MCP /
- gene expression /
- terpenoid

HTML全文

图 1 乙烯和1-MCP处理对白姜花挥发性萜类香气物质的影响

“*”表示与对照差异达到0.05的显著水平(单因素方差分析)

Figure 1. Effects of ethylene and 1-MCP treatments on volatile terpene components of Hedychium coronarium

下载: 全尺寸图片幻灯片

图 2 乙烯和1-MCP处理对白姜花萜类合成酶相关基因表达的影响

“*”和“**”分别表示与对照差异达到0.05和0.01的显著水平(单因素方差分析)

Figure 2. Effects of ethylene and 1-MCP treatments on the expressions of terpene synthase related genes in Hedychium coronarium

下载: 全尺寸图片幻灯片

图 3 乙烯和1-MCP处理对HcEIL1-1和HcEIL1-2表达的影响

“*”和“**”分别表示与对照差异达到0.05和0.01的显著水平(单因素方差分析)

Figure 3. Effects of ethylene and 1-MCP treatments on the expressions of HcEIL1-1 and HcEIL1-2

下载: 全尺寸图片幻灯片

表 1 荧光定量PCR引物

Table 1 Fluorescent quantitative PCR primers

扩增基因种类	基因名称	引物序列(5′→3′)	参考文献
内参基因	GAPDH	F:TAACATCATTCCCAGCAGCACTG	刘洋^[15]
内参基因	GAPDH	R:GTGGATCTCACTGTCAGGCTC	刘洋^[15]
乙烯信号转录因子	HcEIL1-1	F:GAAGTTGGTGGCTTGGTGGAGC	Chen等^[16]
乙烯信号转录因子	HcEIL1-1	R:TCCCTACCATTTCTCTCGAGAGAAC	Chen等^[16]
乙烯信号转录因子	HcEIL1-2	F:GCTTGGTGGAGCAATCTCAGTAC	Chen等^[16]
乙烯信号转录因子	HcEIL1-2	R:CCCTACCATTTCTCTCGAGAGAAC	Chen等^[16]

下载: 导出CSV

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