乙烯和1-MCP对白姜花萜类香气及相关基因表达的影响

刘晓洲; 范燕萍; 余让才; 岳跃冲; 李昕悦; 玉云祎

doi:10.7671/j.issn.1001-411X.2018.04.011

乙烯和1-MCP对白姜花萜类香气及相关基因表达的影响

Effect of ethylene and 1-MCP on the terpenoid fragrance of Hedychium coronarium and the expressions of related genes

摘要

摘要:
目的研究乙烯和1-MCP对白姜花萜类香气物质合成释放的影响，探究相关基因的表达变化。

方法以白姜花Hedychium coronarium为材料，分别用10 μL·L^–1的乙烯和4 μL·L^–1的乙烯抑制剂1-MCP处理8 h，随后采用顶空固相微萃取和气相色谱–质谱(GC-MS)技术进行挥发性香气物质的测定，所得到的离子图用NIST 08数据库进行定性及定量分析。通过荧光定量PCR分析相关基因的表达情况。

结果乙烯处理后白姜花香气成分罗勒烯、沉香醇、别罗勒烯、石竹烯和法尼烯的挥发量显著上升，相关香气萜类合成酶基因HcTPS1、HcTPS3和HcTPS10的表达量分别上升了116%、182%和63%；而用1-MCP处理后，罗勒烯、别罗勒烯和法尼烯的挥发量显著下降，HcTPS1、HcTPS3和HcTPS10基因的表达量分别降低了45%、30%和30%。乙烯处理后，核转录因子HcEIL1-1和HcEIL1-2的表达量分别上升了51%和114%；1-MCP处理后，HcEIL1-1和HcEIL1-2表达量分别下降了30%和60%。

结论外源乙烯可以提升白姜花萜类花香物质的释放量，白姜花乙烯信号通路中的核转录因子HcEIL1-1和HcEIL1-2与萜类花香合成酶基因的表达模式一致。

Abstract:
Objective To investigate the effect of ethylene and 1-methylcyclopropene (1-MCP) treatment on the terpenoid fragrance of Hedychium coronarium and analyze the expressions of related genes.

Method H. coronarium was treated separately with 10 μL·L^–1 ethylene and 4 μL·L^–1 ethylene inhibitor 1-MCP for 8 h. Volatile scent compounds were measured by headspace solid-phase microextraction and gas chromatography-mass spectrometry (GC-MS), and the obtained ion chromatograms were analyzed using NIST 08 database. The expressions of related genes were analyzed by fluorescence quantitative PCR.

Result After ethylene treatment, the emissions of volatile scent compounds ocimene, linalool, allo-ocimene, β-caryophyllene and α-farnesene increased significantly, and the expressions of related terpene synthase genes HcTPS1, HcTPS3 and HcTPS10 increased by 116%, 182% and 63% respectively. After 1-MCP treatment, the emissions of ocimene, allo-ocimene and α-farnesene decreased significantly, and the expressions of HcTPS1, HcTPS3 and HcTPS10 decreased by 45%, 30% and 30% respectively. After ethylene treatment, the expressions of nuclear transcription factors HcEIL1-1 and HcEIL1-2 increased by 1.51 and 2.14 times respectively. After 1-MCP treatment, the expressions of HcEIL1-1 and HcEIL1-2 decreased by 30% and 60% respectively.

Conclusion Exogenous ethylene can increase the emission of terpenoids in H. coronarium. The expression patterns of HcEIL1-1 and HcEIL1-2, the nuclear transcription factors in ethylene signaling pathway, were consistent with the expression pattern of terpenoid synthase genes.

HTML全文

参考文献(24)

施引文献

资源附件(0)