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    王鸿, 黄烈健, 胡峰. 16年生大叶相思高效芽诱导、增殖体系研究[J]. 华南农业大学学报, 2016, 37(5): 91-97. DOI: 10.7671/j.issn.1001-411X.2016.05.016
    引用本文: 王鸿, 黄烈健, 胡峰. 16年生大叶相思高效芽诱导、增殖体系研究[J]. 华南农业大学学报, 2016, 37(5): 91-97. DOI: 10.7671/j.issn.1001-411X.2016.05.016
    shu
    WANG Hong, HUANG Liejian, HU Feng. Efficient bud inducing and proliferation of 16-year-old Acacia auriculiformis plant[J]. Journal of South China Agricultural University, 2016, 37(5): 91-97. DOI: 10.7671/j.issn.1001-411X.2016.05.016
    Citation: WANG Hong, HUANG Liejian, HU Feng. Efficient bud inducing and proliferation of 16-year-old Acacia auriculiformis plant[J]. Journal of South China Agricultural University, 2016, 37(5): 91-97. DOI: 10.7671/j.issn.1001-411X.2016.05.016
    shu

    16年生大叶相思高效芽诱导、增殖体系研究

    Efficient bud inducing and proliferation of 16-year-old Acacia auriculiformis plant

      摘要
    • 摘要:
      目的 充分利用大叶相思Acacia auriculiformis成年优树的优良性状，提高大叶相思优质苗木扩繁效率、加快其良种选育及推广。
      方法 以16年生大叶相思为材料，对外植体消毒方式进行研究，结合外植体及芽诱导培养基的选择，建立大叶相思高效芽诱导体系；对增殖培养基进行筛选，并研究多次继代与增殖倍数的关系。
      结果 于8月取当年生枝条第3~5腋芽的茎段(最优外植体)，选择φ为0.1%升汞和75%乙醇分别处理18 min和15 s最优外植体，接种于最佳芽诱导培养基MS+6-BA 1.0 mg·L-1+蔗糖40 g·L-1，芽诱导率为92.00%。增殖培养基为Ⅱ型培养基(MS+ 6-BA 1.0 mg·L-1 + NAA 0.1 mg·L-1+Ac 0.05 g·L-1+蔗糖30 g·L-1)时增殖倍数高且稳定，7次继代平均增殖倍数为2.63。
      结论 建立的高效组培体系适于大叶相思成年优树的快速扩繁。

       

      Abstract:
      Objective To utilize the superior properties of mature Acacia auriculiformis, improve propagation efficiency, and accelerate breeding and promotion of elite varieties.
      Method A. auriculiformis bud inducing system was established by comparing the disinfection strategies for the explants from 16-year-old A. auriculiformis and screening the bud inducing media. The proliferation media were screened and the relationship between subculture times and proliferation rate was studied.
      Result The optimal explants were those collected from the 3th-5th node of shoots in August. For the optimal explants treated with 0.1% mercuric chloride for 18 min and 75% ethanol for 15 s, and inoculated onto the best bud inducing medium containing MS medium + 6-BA 1.0 mg·L-1+sucrose 40 g·L-1 , the germination rate reached 92.00%. When type Ⅱ medium (MS+ 6-BA 1.0 mg·L-1 + NAA 0.1 mg·L-1+Ac 0.05 g·L-1+sucrose 30 g·L-1) was used for proliferation, the proliferation rate was high and stable with an average rate of 2.63 for seven generations.
      Conclusion This tissue culture system enables fast propagation for mature elite plants of A. auriculiformis.

       

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