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    代晓彦, 李翌菡, 许炜明, 沈祖乐, 黄振, 吴建辉, 任顺祥, 邱宝利. 亚洲柑橘木虱2株高致病力病原真菌菌株的筛选[J]. 华南农业大学学报, 2016, 37(5): 62-65. DOI: 10.7671/j.issn.1001-411X.2016.05.011
    引用本文: 代晓彦, 李翌菡, 许炜明, 沈祖乐, 黄振, 吴建辉, 任顺祥, 邱宝利. 亚洲柑橘木虱2株高致病力病原真菌菌株的筛选[J]. 华南农业大学学报, 2016, 37(5): 62-65. DOI: 10.7671/j.issn.1001-411X.2016.05.011
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    DAI Xiaoyan, LI Yihan, XU Weiming, SHEN Zule, HUANG Zhen, WU Jianhui, REN Shunxiang, QIU Baoli. Screening two entomopathogenic fungal strains with high virulence against Asia citrus psyllid, Diaphorina citri[J]. Journal of South China Agricultural University, 2016, 37(5): 62-65. DOI: 10.7671/j.issn.1001-411X.2016.05.011
    Citation: DAI Xiaoyan, LI Yihan, XU Weiming, SHEN Zule, HUANG Zhen, WU Jianhui, REN Shunxiang, QIU Baoli. Screening two entomopathogenic fungal strains with high virulence against Asia citrus psyllid, Diaphorina citri[J]. Journal of South China Agricultural University, 2016, 37(5): 62-65. DOI: 10.7671/j.issn.1001-411X.2016.05.011
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    亚洲柑橘木虱2株高致病力病原真菌菌株的筛选

    Screening two entomopathogenic fungal strains with high virulence against Asia citrus psyllid, Diaphorina citri

      摘要
    • 摘要:
      目的 筛选对亚洲柑橘木虱Diaphorina citri Kuwayama具有高致病力的真菌菌株，为其微生物农药的研发奠定基础。
      方法 测定5株球孢白僵菌Beauveria bassiana(菌株A~E)和5株玫烟色棒束孢菌Isaria fumosorosea菌株(菌株A~E)的生长速率、产孢量以及对木虱若虫的侵染致死率等，并进行比较分析。
      结果 5株球孢白僵菌培养10 d后产孢量存在较大差异，A菌株的产孢量最少(7.96×107 mL-1)，E菌株的产孢量最多(3.78×108 mL-1)，两者差异显著；在喷施球孢白僵菌孢子1×106 mL-1 7 d后，对柑橘木虱低龄若虫的致死率与对照差异不显著，以A菌株的致死率最高(78.0%)，E菌株次之(77.0%)。5株玫烟色棒束孢菌株培养10 d后，B菌株产孢量最多(6.04×108 mL-1)，E菌株的最少(6.28×107 mL-1)，两者差异显著；在喷施玫烟色棒束孢孢子1×106 mL-1 7 d后，除D菌株外，其他制剂对亚洲柑橘木虱低龄若虫的致死率均显著大于对照，B菌株的致死率最高(81.0%)。
      结论 综合考虑产孢量及致病力等指标，球孢白僵菌E菌株和玫烟色棒束孢B菌株是具有良好研发前景的柑橘木虱生物防治高致病力优良菌株。

       

      Abstract:
      Objective To screen entomopathogenic fungal strains with high virulence against Asia citrus psyllid (ACP), Diaphorina citri Kuwayama, and to provide a basis for development of microbial pesticide against ACP.
      Method The growth and sporulation rates of five Beauveria bassiana strains numbered A to E and five Isaria fumosorosea strains numbered A to E, and the mortality rates of ACP after infected by these ten fungal strains were investigated.
      Result The spores quantity of five B. bassiana strains were significantly different after cultured for 10 days, strain E had the largest spores quantity(3.78×108 mL-1) and strain A had the least spores quantity(7.96×107 mL-1). There was significant difference of spores quantity between strain E and strain A. There was no significant difference among the mortality rates of 2-3 instar ACP nymphs seven days after the spray of 1×106 mL-1 B. bsssiana fungal spores, and the highest mortality rate (78.0%) was found in the strain A treatment, followed by the strain E treatment (77.0%). For five I. fumosorosea strains, the spores quantity after cultured for 10 days, strain B had the highest sporulation rate (6.04×108 mL-1) and strain E had the least spores quantity(6.28×107 mL-1). There was significant difference of spores quantity between strain B and strain E. The mortality rates of 2-3 instar ACP nymphs seven days after the spray of 1×106 mL-1 I. fumosorosea fungal spores were higher than the control group except strain D. ACP infected by strain B had the highest mortality rate(81.0%).
      Conclusion B. bassiana strain E and I. fumosorosea strain B are considered with high virulence against ACP and can be potentially used for biological control of ACP.

       

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