Effects of heat stress on antioxidant capacity and gastrocnemius musclefiber types of broilers
-
摘要:目的
研究慢性热应激对黄羽肉鸡肝脏和肌肉的抗氧化能力与内质网应激以及腓肠肌肌纤维类型的影响.
方法试验选用20只47日龄黄羽肉鸡，随机分为常温组和慢性热应激组，每组10只.鸡只饲养在人工气候室，试验期30 d.试验结束采集血液、肝脏和腓肠肌样品，利用试剂盒法检测血清生化指标、血清激素以及血清和肝脏抗氧化指标.利用实时荧光定量PCR法检测内质网应激（Endoplasmic reticulum stress, ERS）相关基因和腓肠肌不同肌纤维肌球蛋白重链（Myosin heavy chain, MyHC）基因mRNA的表达.
结果和结论慢性热应激显著降低肉鸡平均日增质量、血清甘油三酯（TG）、甲状腺激素（T3和T4）、胰岛素样生长因子1（Insulin-like growth factor 1, IGF-1），显著升高血清皮质酮（CORT）水平（P < 0.05）.同时，慢性热应激显著升高血清中丙二醛（MDA）含量，降低血清和肝脏谷胱甘肽过氧化物酶（GSH-PX）活性和肝脏总抗氧化能力（T-AOC）（P < 0.05）.此外，慢性热应激显著提高肉鸡肝脏和腓肠肌中热休克蛋白70（Heat shock protein 70, HSP70）基因以及腓肠肌中转录激活因子4（Activating transcription factor 4, ATF4）基因mRNA的表达（P < 0.05），降低腓肠肌中葡萄糖调节蛋白78（Glucose-regulated protein 78, GRP78）基因mRNA的表达，而对肝脏中ATF4和GRP78基因mRNA的表达量无显著影响.最后，慢性热应激显著降低腓肠肌慢肌（Slow myofiber，SM）MyHC基因mRNA表达量（P < 0.05）而对快红肌（Fast red myofiber, FRM）和快白肌（Fast white myofiber, FWM）MyHC基因mRNA表达量无影响.研究结果提示，慢性热应激可能通过降低血清生长和代谢激素、机体抗氧化能力以及引发细胞内质网应激影响肉鸡生长和腓肠肌纤维类型.
Abstract:ObjectiveThe present study was conducted to investigate the effects of chronic heat stress on antioxidant capacity, endoplasmic reticulum stress and gastrocnemius muscle fiber types of yellow feather broilers.
MethodTwenty 47-day-old yellow feather broilers were randomly divided into control and chronic heat stress groups with 10 broilers in each group. The broilers were raised in an artificial climate chamber for 30 days. At the end of the trial, the samples of blood, liver and gastrocnemius muscle were collected. Serum biochemical indexes, hormones and antioxidant indexes of liver and gastrocnemius muscle were determined using the commerical assay kit method. And the mRNA expression of endoplasmic reticulum stress (ERS) related genes and myosin heavy chain (MyHC) of different skeletal muscle fiber types were detected using real-time quantitative PCR.
Result and conclusionChronic heat stress significantly (P < 0.05) decreased the average daily gain, serum TG content, thyroid hormone (T3 and T4), and insulin-like growth factor 1 (IGF-1) levels, and increased serum corticosterone (CORT) concentration. Meanwhile, chronic heat stress markedly (P < 0.05) enhanced serum malondialdehyde (MDA) content, and reduced serum, GSH-PX activity and total antioxidant capacity (T-AOC) in liver.In addition, chronic heat stress remarkablly (P < 0.05) elevated the mRNA expression of heat shock protein 70 (HSP70) in liver and gastrocnemius muscle, and of activating transcription factor 4 (ATF4) in gastrocnemius muscle, and decreased the glucose-regulated protein 78 (GRP78) mRNA expression in gastrocnemius muscle, with no obvious effect on the mRNA expression of ATF4 and GRP78 in liver. Moreover, chronic heat stress significantly (P < 0.05) inhibited the mRNA expression of slow myofiber MyHC, with no influence on MyHC mRNA expression of fast red myofiber and fast white myofiber. In conclusion, this results suggest that chronic heat stress may affect broiler growth and gastrocnemius muscle fiber types by decreasing serum hormones involved in growth and metabolism, antioxidant capacity and eliciting ERS.
-
-
![]()
图 1 慢性热应激对肉鸡肝脏（A）和腓肠肌（B）HSP70、ATF4和GRP78基因mRNA表达的影响
*表示与对照组相比差异达0.05显著水平（t检验）.
Figure 1. Effects of chronic heat stress on the mRNA expressions of HSP70, ATF4 and GRP78 genes in liver (A) and gastrocnemius muscle (B) of broilers
![]()
图 2 慢性热应激对肉鸡腓肠肌不同肌纤维类型肌球蛋白重链基因mRNA表达的影响
*表示与对照组相比差异达0.05显著水平（t检验）.
Figure 2. Effects of chronic heat stress on the mRNA expressions of different types of myosin heavy chain in gastrocnemius muscle of broilers
表 1 47 ~ 77日龄黄羽肉鸡基础饲粮组成及营养水平1）
Table 1 Composition and nutrient levels of basal diets for yellow feather broilers aged from 47 to 77 days
下载: 导出CSV
表 3 慢性热应激对黄羽肉鸡生长、血液生化指标以及血清激素水平的影响1）
Table 3 Effects of chronic heat stress on growth, serum biochemical indexes, and serum hormone levels of yellow feather broilers
下载: 导出CSV
表 4 慢性热应激对黄羽肉鸡血清和肝脏抗氧化指标的影响1）
Table 4 Effects of chronic heat stress on serum and liver antioxidant indexes of yellow feather broilers
下载: 导出CSV
-
[1] 田书音, 李冰.热应激对肉鸡生产的危害及抗应激添加剂的研究[J].饲料博览, 2010(10): 10-13. doi: 10.3969/j.issn.1001-0084.2010.10.003 [2] LAI E, TEODORO T, VOLCHUK A.Endoplasmic reticu- lum stress: Signaling the unfolded protein response[J]. Physiology (Bethesda), 2007, 22(3): 193-201.
[3] 刘宝琴, 王华芹.内质网应激与未折叠蛋白反应的研究进展[J].中华肿瘤防治杂志, 2010, 17(11): 869- 872. http://d.old.wanfangdata.com.cn/Periodical/wswxzz201702017 [4] CAO S S, KAUFMAN R J.Unfolded protein response [J]. Curr Biol, 2012, 22(16): R622-R626. doi: 10.1016/j.cub.2012.07.004
[5] 邬理洋, 黄兴国.肌纤维类型对肉质品质的影响[J].湖南饲料, 2010(3): 21-23. doi: 10.3969/j.issn.1673-7539.2010.03.013 [6] LEE S H, JOO S T, RYU Y C.Skeletal muscle fiber type and myofibrillar proteins in relation to meat quality[J]. Meat Sci, 2010, 86(1): 166-170. doi: 10.1016/j.meatsci.2010.04.040
[7] 杨培歌. 热应激对肥育猪肌肉品质及其代谢物的影响[D]. 北京: 中国农业科学院, 2014. http://cdmd.cnki.com.cn/Article/CDMD-82101-1014326613.htm [8] LARA L J, ROSTAGNO M H.Impact of heat stress on poultry production[J]. Animals, 2013, 3(2): 356-369. doi: 10.3390/ani3020356
[9] 王士长, 陈静, 黄怡, 等.热应激对肉鸡生产性能和血清生化指标的影响[J].中国家禽, 2007, 29 (15): 11-13. doi: 10.3969/j.issn.1004-6364.2007.15.003 [10] 李军乔. 高温环境对肉仔鸡血液生化指标、热应激蛋白(HSP72)转录及肉品质的影响[D]. 保定: 河北农业大学, 2004. http://cdmd.cnki.com.cn/article/cdmd-11920-2004091510.htm [11] 刘铀, 林红英, 罗东君, 等.热应激对肉鸡血液生化指标及内分泌机能的影响[J].湛江海洋大学学报, 1999, 19(1): 61-64. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK199900576437 [12] 何世山, 金小军.高温对肉鸡血液生化指标的影响[J].浙江大学学报:农业与生命科学版, 2003, 29 (3): 77-80. http://d.old.wanfangdata.com.cn/Periodical/zjdxxb-nyysm200303017 [13] 李文立, 路静, 孙振钧, 等.谷氨酰胺对热应激肉鸡抗氧化性能的影响[J].动物营养学报, 2011, 23(4): 695-702. doi: 10.3969/j.issn.1006-267x.2011.04.022 [14] 林海, 杜荣.热应激对肉鸡组织过氧化状态的影响[J].动物营养学报, 2001, 13(2): 30-32. doi: 10.3969/j.issn.1006-267X.2001.02.007 [15] JANG I S, KO Y H, MOON Y S, et al.Effects of vitamin C or E on the Pro-inflammatory cytokines, heat shock pro- tein 70 and antioxidant status in broiler chicks under sum- mer conditions[J]. Asian Australas J Anim Sci, 2014, 27(5): 749-756. doi: 10.5713/ajas.2013.13852
[16] 王立, 姜建阳, 任慧英, 等.两种铬源对热应激肉鸡抗氧化性能的影响[J].饲料广角, 2010(9): 26-27. doi: 10.3969/j.issn.1002-8358.2010.09.008 [17] 孙培明. 肉鸡热应激损伤与热休克蛋白70表达的研究[D]. 南京: 南京农业大学, 2006. http://cdmd.cnki.com.cn/Article/CDMD-10307-2007010246.htm [18] 李娟. 鸡生长发育和肌纤维生长的影响因素与相关基因表达研究[D]. 雅安: 四川农业大学, 2013. http://cdmd.cnki.com.cn/Article/CDMD-10626-1014103189.htm [19] 杨小娇, 许静, 宗凯, 等.不同温度热应激对肉鸡血液生化指标及肉品质的影响[J].家禽科学, 2011(3): 10-14. doi: 10.3969/j.issn.1673-1085.2011.03.004 [20] YAMAGUCHI T, SUZUKI T, ARAI H, et al.Continuous mild heat stress induces differentiation of mammalian myo- blasts, shifting fiber type from fast to slow[J]. Am J Physiol-Cell Ph, 2010, 298(1): C140-C148. doi: 10.1152/ajpcell.00050.2009
[21] YAMAGUCHI T, OMORI M, TANAKA N, et al.Distinct and additive effects of sodium bicarbonate and continuous mild heat stress on fiber type shift via calcineurin /NFAT pathway in human skeletal myoblasts[J]. Am J Physiol- Cell Ph, 2013, 305(3): C323-C333. doi: 10.1152/ajpcell.00393.2012
计量
- 文章访问数: 1418
- HTML全文浏览量: 4
- PDF下载量: 1709
