Objective To research the effects of the biological organic fertilizer(BOF) on controlling wax gourd wilt disease, microorganism and enzyme activities in soil.

Method The biological organic fertilizer(BOF) was made by mixing the mature compost with functional bacteria in proportion to identify the effects in pot experiment.

Result and conclusion The occurrence of wax gourd wilt disease decreased by applying BOF, and along with the increasing amount of fertilizer application, the efficacy was more significant. When the disease-control effect of CPC treatment was assigned as 0, the disease-control effects of the treatments of BOF1, BOF2 and BOF3 were 42.27%, 52.92% and 70.15%, respectively, which were higher than that of disease-control effect(31.91%)of organic fertilizer treatment; With the same amount of effective nutrients, the height, stem diameter, leaf width, and fresh mass of plant in BOF2 treatment were significantly higher than that of OF treatment, which indicated that the BOF could promote the growth of wax gourd. The number of bacteria and actinomycets in soil increased significantly by BOF, while the amount of fungi and Fusarium oxysporum in soil decreased. Urease, acid phosphatase, catalase and invertase activities in soil of BOF were higher than those of CK and CPC treatment. The results showed that BOF could improve soil enzyme activities, adjust soil microbial community structure, improve the soil quality and fertility conditions, hence decrease the occurrence of the wax gourd wilt disease.