鸭胸腺内 Ghrelin 免疫组织化学的定位分布及发育性变化

    Immunohistochemical localization and developmental changes of ghrelin in the duck thymus

    • 摘要: 【目的】研究 Ghrelin 在鸭胸腺发育过程中的定位分布及变化规律,以期为进一步探讨 Ghrelin 对胸腺生理功能的影响提供形态学资料.【方法】选用不同发育阶段的天府肉鸭,胚胎期分7个组,即:14、16、18、20、22、24和26 d胚龄组;胚后期分12个组,即:新生雏、1、3、5、8、11、14、17、20、26、29和32周龄组.运用免疫组织化学技术及形态计量方法研究 Ghrelin 在鸭胸腺发育过程中的定位分布及变化规律.【结果和结论】Ghrelin 免疫阳性细胞弥散分布于20 d胚龄至胚后32周龄的胸腺小叶皮质和髓质;阳性细胞呈圆形、卵圆形、星形或不规则形,主要为胸腺上皮细胞和巨噬细胞.阳性细胞数量在胚胎期无明显差异,在胚后胸腺继续发育期(新生雏~8周龄)和成熟期(11~17周龄)呈波浪状变化,在退化期(20~32周龄)呈下降趋势.说明在天府肉鸭胸腺个体发育中存在 Ghrelin 免疫阳性细胞,其数量表现出明显增龄变化特性,与胸腺退化密切相关.

       

      Abstract: 【Objective】 The immunohistochemical localization and developmental changes of ghrelin in the duck thymus were studied to provide the morphological effect of ghrelin on the physiological function of thymus. 【Method】 Tianfu ducks were divided into 19 groups as follows: 7 groups in the embryonic period (14, 16, 18, 20, 22, 24 and 26 days at embryonic stage), 12 groups after hatching (at neonatal stage, 1, 3, 5, 8, 11, 14, 17, 20, 26, 29 and 32 weeks). The immunohistochemical method associated with morphometric analysis was used. 【Result and conclusion】 The ghrelin immunoreactivity (-ir) cells with round, ovoid, stellate and irregular aspects were maily thymic epitheilial cells and macrophages, and diffusely distributed in the thymic cortex and medulla from embryonic 20 days to 32 weeks after hatching. The number of ghrelin-ir cells in the thymic cortex and medulla was unchanged during the embryonic development, presenting a wave-changed pattern during the continuous development (from neonatal stage to 8 weeks after hatching) and matured periods (from 11 to 17 weeks after hatching ), which decreased during the involution stage (from 20 to 32 weeks after hatching). These results suggested that ghrelin-ir cells existed in the duck thymus and their numbers exhibited obvious age-related changes, which can be closely related to the involution of the duck thymus.

       

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